Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 19.L2, Problem 14CT
14. Eighty-six people at a St. Patrick's Day celebration developed diarrhea, cramps, and vomiting after eating a traditional dinner of corned beef, cabbage, potatoes, and ice cream. Symptoms appeared within 10 hours of eating, on average. Of those afflicted, 85 had eaten corned beef and one had not. The corned beef had been cooked in an oven the day before the event, stored in the refrigerator, and sliced and placed under heat lamps 11/2 hours before serving.
a. Use this information to diagnose the food poisoning agent.
b. What sorts of lab tests could one do to verify it as this bacterium?
c. What would you have done to prevent this incident?
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 19 Solutions
Foundations in Microbiology
Ch. 19.1 - 1. Describe how cellular characteristics are used...Ch. 19.1 - 1. Explain why Bacillus, Clostridium, and...Ch. 19.2 - 2. Recall the general characteristics of the genus...Ch. 19.2 - 3. Distinguish between cutaneous and pulmonary...Ch. 19.2 - 4. State the general characteristics of the genus...Ch. 19.2 - 5. Recall the organisms responsible for...Ch. 19.2 - Prob. 6ELOCh. 19.2 - Prob. 7ELOCh. 19.2 - Prob. 8ELOCh. 19.2 - 9. Compare food intoxication caused by Bacillus...
Ch. 19.2 - Prob. 2CYPCh. 19.2 - Prob. 3CYPCh. 19.2 - 4. What are the common elements of puncture...Ch. 19.2 - 5. What is the relationship between the normal...Ch. 19.2 - Prob. 6CYPCh. 19.2 - Prob. 7CYPCh. 19.2 - 8. ln what way is the ingested agent responsible...Ch. 19.3 - 10. Relate the severity of listeriosis with the...Ch. 19.3 - 11. Explain why people in certain occupations are...Ch. 19.3 - 9. Compare the effects of listeriosis in healthy...Ch. 19.3 - 10. Why do erysipeloids commonly appear on the...Ch. 19.4 - Prob. 12ELOCh. 19.4 - Prob. 13ELOCh. 19.4 - Prob. 11CYPCh. 19.4 - Prob. 12CYPCh. 19.5 - Prob. 14ELOCh. 19.5 - Prob. 15ELOCh. 19.5 - Prob. 16ELOCh. 19.5 - Prob. 17ELOCh. 19.5 - 18. Explain the significance of nontuberculous...Ch. 19.5 - Prob. 13CYPCh. 19.5 - 14. Compile a list of the advantages,...Ch. 19.5 - 15. Explain how and why antibacterial treatment...Ch. 19.5 - 16. List several differences between lepromatous...Ch. 19.5 - Prob. 17CYPCh. 19.5 - 18. List the diseases and at-risk populations...Ch. 19.6 - Prob. 19ELOCh. 19.6 - 20. Describe the types of infections attributable...Ch. 19.6 - 19. Compare the types of infections caused by the...Ch. 19.L1 - 1. What is/are the usual habitat(s) of...Ch. 19.L1 - Prob. 2MCQCh. 19.L1 - Prob. 3MCQCh. 19.L1 - 4. Clostridium perfringens causes a. myonecrosis...Ch. 19.L1 - Prob. 5MCQCh. 19.L1 - Prob. 6MCQCh. 19.L1 - Prob. 7MCQCh. 19.L1 - Prob. 8MCQCh. 19.L1 - Prob. 9MCQCh. 19.L1 - 10. Soil mycobacteria can be the cause of a....Ch. 19.L1 - Prob. 11MCQCh. 19.L1 - Prob. 12MCQCh. 19.L1 - Prob. 13MCQCh. 19.L1 - Prob. 14MCQCh. 19.L1 - Prob. 15MCQCh. 19.L1 - 16. Matching. Match the disease with the principal...Ch. 19.L1 - Prob. 1CSRCh. 19.L1 - 2. During this outbreak, some people sickened with...Ch. 19.L1 - 3. No listeria monocytogenes was discovered in the...Ch. 19.L1 - Prob. 1WCCh. 19.L1 - Prob. 2WCCh. 19.L1 - Prob. 3WCCh. 19.L1 - Prob. 4WCCh. 19.L1 - Prob. 5WCCh. 19.L1 - 6. a. Why is listeriosis a serious problem even...Ch. 19.L1 - Prob. 7WCCh. 19.L1 - Prob. 8WCCh. 19.L1 - 9. a. Outline the unique characteristics of...Ch. 19.L1 - Prob. 10WCCh. 19.L1 - 11. a. What is the importance of NTM? b. Describe...Ch. 19.L1 - Prob. 12WCCh. 19.L2 - Prob. 1CTCh. 19.L2 - 2. a. Why is it unlikely that diseases such as...Ch. 19.L2 - Prob. 3CTCh. 19.L2 - Prob. 4CTCh. 19.L2 - Prob. 5CTCh. 19.L2 - 6. Adequate cooking is the usual way to prevent...Ch. 19.L2 - 7. a. Why do patients who survive tetanus and...Ch. 19.L2 - Prob. 8CTCh. 19.L2 - 9. How can one tell that acne involves an...Ch. 19.L2 - Prob. 10CTCh. 19.L2 - Prob. 11CTCh. 19.L2 - Prob. 12CTCh. 19.L2 - 13. Which diseases discussed in this chapter have...Ch. 19.L2 - 14. Eighty-six people at a St. Patrick's Day...Ch. 19.L2 - 15. An outbreak of gastrointestinal illness was...Ch. 19.L2 - Prob. 1VCCh. 19.L2 - 2. From chapter 3, figure 3.8. What type of...
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