The reason for the use of silica gel columns with smaller particle sizes in chromatographic separation should be stated. Concept introduction: Liquid chromatography is used for purification of high boiling compounds. It is known as column chromatography. In liquid chromatography, column is packed by stationary phase. Usually, a solid adsorbent with a liquid coated on it is used as stationary phase. Often a pure liquid or solution of liquids composes the mobile phase. It is also called the elution solvent. The elution solvent comes down the column under the influence of gravity. Selective interactions are responsible for separation of the sample among stationary and mobile phase. The sequence of elution of compounds from sample depends on the polarity of mobile and stationary phases.

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Chapter 19, Problem 5Q

Interpretation Introduction

Interpretation:

The reason for the use of silica gel columns with smaller particle sizes in chromatographic separation should be stated.

Concept introduction:

Liquid chromatography is used for purification of high boiling compounds. It is known as column chromatography. In liquid chromatography, column is packed by stationary phase. Usually, a solid adsorbent with a liquid coated on it is used as stationary phase. Often a pure liquid or solution of liquids composes the mobile phase. It is also called the elution solvent.

The elution solvent comes down the column under the influence of gravity. Selective interactions are responsible for separation of the sample among stationary and mobile phase. The sequence of elution of compounds from sample depends on the polarity of mobile and stationary phases.

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