Laboratory Techniques in Organic Chemistry
Laboratory Techniques in Organic Chemistry
4th Edition
ISBN: 9781464134227
Author: Jerry R. Mohrig, David Alberg, Gretchen Hofmeister, Paul F. Schatz, Christina Noring Hammond
Publisher: W. H. Freeman
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Chapter 19, Problem 2Q
Interpretation Introduction

Interpretation:

The precautions that must be taken when the mixture of compounds is introduced onto the liquid chromatography adsorbent column should be explained.

Concept introduction:

Liquid chromatography is used for purification of high boiling compounds. It is known as column chromatography. In liquid chromatography, column is packed by stationary phase. Usually a solid adsorbent with a liquid coated on it is used as stationary phase. Often a pure liquid or solution of liquids compose the mobile phase. It is also called the elution solvent.

The elution solvent comes down the column under the influence of gravity. Selective interactions are responsible for separation of the sample among stationary and mobile phase. The sequence of elution of compounds from sample depends on the polarity of mobile and stationary phase.

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Laboratory Techniques in Organic Chemistry

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