Campbell Biology 11th Edition - Valuepack
11th Edition
ISBN: 9780134833545
Author: Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece Neil A. Campbell Lisa A. Urry
Publisher: PEARSON
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Chapter 19, Problem 4TYU
Summary Introduction
Introduction: Bacteria are single-celled prokaryotic microorganisms. A bacteriophage is also called as bacterial virus that is formed when a virus infects a bacterium. T2 and T4 are enterobacteria phages that infect and kill E. coli. They are also known as T-even phages because they are double stranded. T-even phages are commonly used as model organisms for various genetic studies. The experiments performed by Hershey and Chase using bacteriophages already proved that DNA is the genetic material of inheritance.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 19 Solutions
Campbell Biology 11th Edition - Valuepack
Ch. 19.1 - Prob. 1CCCh. 19.1 - MAKE CONNECTIONS Bacteriophages were used to...Ch. 19.2 - Compare the effect on the host cell of a lytic...Ch. 19.2 - MAKE CONNECTIONS Compare the CRISPR-Cas system to...Ch. 19.2 - Prob. 3CCCh. 19.2 - Why is HIV called a retrovirus?Ch. 19.2 - Prob. 5CCCh. 19.3 - Describe two ways in which a preexisting virus can...Ch. 19.3 - Contrast horizontal and vertical transmission of...Ch. 19.3 - WHAT IF? TMV has been isolated from virtually all...
Ch. 19 - Are viruses generally considered living or...Ch. 19 - Prob. 19.2CRCh. 19 - Prob. 19.3CRCh. 19 - Which of the following characteristics,...Ch. 19 - Emerging viruses arise by (A) mutation of existing...Ch. 19 - Prob. 3TYUCh. 19 - Prob. 4TYUCh. 19 - Prob. 5TYUCh. 19 - DRAW IT Redraw Figure 19.8 to show the replicative...Ch. 19 - EVOLUTION CONNECTION The successof some viruses...Ch. 19 - SCIENTIFIC INQUIRY When bacteria infect an animal,...Ch. 19 - WRITE ABOUT A THEME: ORGANIZATION While viruses...Ch. 19 - Prob. 10TYU
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