Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 19, Problem 3CHI
You have recently established a pure culture of a new archaeon from soil. Describe the approaches you would use to identify your new microbe to the species level.
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The techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to:
Staining the unknown for initial characterization by microscopy.
Using a dichotomous key strategy to systematically rule out other organisms.
Testing the organism for key biochemical traits.
What is the importance of using a test/control organism in describing the cultural characteristics of an unknown bacterium?
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is one experiment you could do, involving culturing the organism?
Chapter 19 Solutions
Prescott's Microbiology
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - What is polyphasic taxonomy, and what three types...Ch. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - MICRO INQUIRY Would this curve be shifted to the...Ch. 19.3 - Why does isolate #2 yield only one DNA fragment...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is it not safe to assume that two...
Ch. 19.3 - Prob. 3RIACh. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 5RIACh. 19.4 - Could a phylotype be considered an OTU? What about...Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3RIACh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Which do you think would have a pan-genome more...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4RIACh. 19.6 - Why is the second edition of Bergeys Manual no...Ch. 19.6 - Describe two different situations in which it...Ch. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - Bacteria and Archaea were classified phenetically...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 4CHICh. 19 - Prob. 5CHICh. 19 - In 2007 a severe food-borne outbreak of...Ch. 19 - Prob. 7CHI
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?arrow_forwardIs it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What type of name should a microorganism have if it has been well identified but cannot yet be cultured in isolation?arrow_forwardThe process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaarrow_forward
- Please make an aseptic technique and pure culture technique flowchartarrow_forwardDefine the term sterile. What two methods did Pasteur used tomake solutions sterile?arrow_forwardA veterinarian suspects that a new disease of house cats is caused by a specific type of bacterium. Describe a set of procedures for providing this hypothesis. EXAMPLE GIVEN ON THE IMAGE.arrow_forward
- The use of culture-independent techniques has increased our understanding of microbial diversity without cultivation. Therefore, do microbiologists still need to attempt to grow new microbial species? Explain.arrow_forwardYou are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 9) What makes the bacteria Gram negative?10) Approximately how long would it take you to finish the entire procedurearrow_forwardYou are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**arrow_forward
- Compare and contrast three common laboratory techniques forseparating bacteria in a mixed sample.arrow_forwardA soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.arrow_forwardexplain why you would expect to see similarities in the types of colonies on the fomite plates and the handwashing plates?arrow_forward
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