Chapter 18.5, Problem 3MI

Summary Introduction

In tandem mass spectrometry, the collection of protein fragments is run through the two mass spectrometers in a sequential manner. This forms the difference between the mass spectrometry and tandem mass spectrometry. In this method, the first spectrometer separates the proteins and fragments which are to be fragmented further. Then, the second spectrometer determines the amino acid sequence of each of the smaller fragments on the basis of the molecular weight. Thus, this technique uses two mass analyzers that are combined in series in order to select the protein.