11th Edition

ISBN: 9781264731060

Author: WILLEY

Publisher: MCG

Concept explainers

Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology …

DNA Fingerprinting and Gel Electrophoresis

The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological sampl…

Molecular Markers

A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the fi…

DNA Sequencing

The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under …

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Textbook Question

Chapter 18.5, Problem 3CC

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Compare and contrast metagenomics with metatranscriptomics.

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Chapter 18.5, Problem 2CC

Chapter 18.5, Problem 4CC

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Need bioinformatics help. 1) Examine the quality of the raw sequencing data 2) Trim low quality sequences and sequencing adapters from the raw reads 3) Align trimmed reads against the reference genome 4) Count number of reads mapped per annotated gene 5) Differential expression analysis 6) Visualize sequencing reads aligned to the reference genome 7) Gene set enrichment analysis (GSEA) Choices: Google Colab UCSC Genome Browser fasterq-dump MultiQC BLAST DESeq2 STAR Gorilla featureCounts SNPEff samtools flagstat BWA mem Integrative Genome Viewer (IGV) LoFreq Fastp FastQC align.seq 2) Trim low quality sequences and sequencing adapters from the raw reads 3) Align trimmed reads against the reference genome 4) Count number of reads mapped per annotated gene 5) Differential expression analysis 6) Visualize sequencing reads aligned to the reference genome 7) Gene set enrichment analysis (GSEA) Choices: Google Colab UCSC Genome Browser…

Compare 2D polyacrylamide gel electrophoresis to LC-MS as a proteomics technique to identify differences between patients with a disease and controls without the disease.

Autodock, Gold and Glide are other bioinformatics tools that are widely utilized by many researchers nowadays especially in drug discovery. Briefly explain their applications in this area of research.

Chapter 18 Solutions

PRESCOTT'S MICROBIO W/PROCTORIO

Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4CC Ch. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...Ch. 18.3 - Retrieve, Infer, Apply Examine figure 18.8. How...

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Retrieve, Infer, Apply Compare and contrast metagenomics with metatranscriptomics.

Solution Summary: The author explains the difference between metagenomics and metatranscriptomics.

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Chapter 18 Solutions