Chapter 18, Problem 22P

Scientists now routinely use CRISPR/Cas9 to make defined deletions of a gene that can remove several kb of DNA from the genome. This method is possible even in cells defective in homologous recombination, as long as the cells can still perform nonhomologous end-joining (NHEJ).

a.	How could researchers make such deletions?
b.	A GM animal that may be approved for human consumption by the time this book is published is a “supermuscly” pig made by inactivation of the myostatin gene. During normal development, Myostatin protein prevents the overgrowth of muscles. Given your answer to part (a), how could the supermuscly pig have been generated?