Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 17.3, Problem 9AYP
Summary Introduction
To summarize:
Two genotypic methods that involve DNA analysis.
Concept introduction:
Identification tests are necessary to identify the causative agent of an infectious disease in order to treat the disease. There are various categories used to identify the microbes. These include
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Chapter 17 Solutions
Microbiology: A Systems Approach
Ch. 17.1 - Prob. 1AYPCh. 17.1 - Prob. 2AYPCh. 17.1 - Prob. 3AYPCh. 17.1 - Prob. 4AYPCh. 17.2 - Summarize the most common methods used for direct...Ch. 17.2 - Prob. 6AYPCh. 17.2 - Prob. 7AYPCh. 17.3 - Prob. 2CFCh. 17.3 - Prob. 8AYPCh. 17.3 - Prob. 9AYP
Ch. 17.3 - Prob. 10AYPCh. 17.3 - Prob. 11AYPCh. 17.3 - Differentiate between sensitivity and specificity.Ch. 17.3 - Explain how fluorescent antibodies can be used in...Ch. 17.3 - Prob. 14AYPCh. 17.4 - Prob. 15AYPCh. 17.4 - Prob. 16AYPCh. 17.4 - Prob. 17AYPCh. 17.5 - Prob. 18AYPCh. 17.5 - List the major advantages of microarray methods of...Ch. 17.5 - Summarize the benefits of using whole-genome...Ch. 17.5 - Prob. 21AYPCh. 17 - Prob. 1CFCh. 17 - Prob. 1MCQCh. 17 - Prob. 2MCQCh. 17 - Prob. 3MCQCh. 17 - Prob. 4MCQCh. 17 - Prob. 5MCQCh. 17 - Prob. 6MCQCh. 17 - Prob. 7MCQCh. 17 - Prob. 8MCQCh. 17 - The Western blot test can be used to identify a....Ch. 17 - Prob. 10MCQCh. 17 - True-False Questions. If the statement is true,...Ch. 17 - Prob. 12TFCh. 17 - Prob. 13TFCh. 17 - Prob. 14TFCh. 17 - Prob. 15TFCh. 17 - Explain why specimens should be taken aseptically,...Ch. 17 - Prob. 2CTQCh. 17 - Prob. 3CTQCh. 17 - Prob. 4CTQCh. 17 - Prob. 5CTQCh. 17 - Prob. 6CTQCh. 17 - Prob. 7CTQCh. 17 - Prob. 8CTQCh. 17 - Prob. 9CTQCh. 17 - Prob. 10CTQCh. 17 - Prob. 1CCCh. 17 - Prob. 2CCCh. 17 - Prob. 3CCCh. 17 - Prob. 4CCCh. 17 - Prob. 1VCCh. 17 - Prob. 2VCCh. 17 - Prob. 1CM
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- Design a grafting experiment to determine if limb mesoderm determines forelimb / hindlimb identity. Include the experiment, a control, and an interpretation in your answer.arrow_forwardThe Snapdragon is a popular garden flower that comes in a variety of colours, including red, yellow, and orange. The genotypes and associated phenotypes for some of these flowers are as follows: aabb: yellow AABB, AABb, AaBb, and AaBB: red AAbb and Aabb: orange aaBB: yellow aaBb: ? Based on this information, what would the phenotype of a Snapdragon with the genotype aaBb be and why? Question 21 options: orange because A is epistatic to B yellow because A is epistatic to B red because B is epistatic to A orange because B is epistatic to A red because A is epistatic to B yellow because B is epistatic to Aarrow_forwardA sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation? The cell membranes are more resistant than normal. An isotonic solution had been added instead of water. A solution of 0.1 M NaCl had been added instead of water. Not enough water had been added to the red blood cell pellet. The man had sickle-cell anaemia.arrow_forward
- A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation? The cell membranes are more resistant than normal. An isotonic solution had been added instead of water. A solution of 0.1 M NaCl had been added instead of water. Not enough water had been added to the red blood cell pellet. The man had sickle-cell anaemia.arrow_forwardWith reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWith reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forward
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