Chapter 17, Problem 5CTQ

a.

Summary Introduction

To describe:

The type of ELISA that can be used to determine an individual’s past exposure to pathogen.

Concept introduction:

ELISA is the acronym for “Enzyme-Linked Immunosorbent Assay”. It is a test that involves use of an antibody which is an enzyme-linked indicator that observes antibody-antigen interactions. It involves usage of a plastic microtiter plate, which provides a surface for the antibodies and antigens to bind or form complex. Enzyme substrate labeling helps in the final detection of the target molecule (antigen/antibody).

b.

Summary Introduction

To describe:

Whether immunochromatographic assays are indirect or direct methods of testing.

Concept introduction:

Immuochromatographic tests are done to confirm the presence of antibodies in a sample, which are specific to an antigen released by a pathogen. In this test, an antigen is placed on a cartridge and the serum sample is placed on the tip of its filter paper. When the serum sample moves on the filter paper, it meets the antigen and produces a color, which shows that the result is positive.