EBK VISUAL ESSENTIALS OF ANATOMY & PHYS
1st Edition
ISBN: 9780321849588
Author: Nath
Publisher: VST
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Chapter 17.3, Problem 1.5SR
Summary Introduction
To match: Each lettered term with the most closely related description.
Introduction:
The urinary bladder is a muscular pouch that is present just superior and posterior to the pubic bone. It stores urine temporarily and controls urination.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 17 Solutions
EBK VISUAL ESSENTIALS OF ANATOMY & PHYS
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Trace the pathway of blood flow through a kidney,...Ch. 17.1 - Prob. 1.1RCh. 17.1 - Prob. 1.2RCh. 17.1 - Prob. 1.3RCh. 17.1 - Prob. 2.1RCh. 17.1 - Prob. 2.2RCh. 17.1 - Prob. 2.3RCh. 17.1 - Prob. 3.1R
Ch. 17.1 - Prob. 3.2RCh. 17.1 - Prob. 3.3RCh. 17.1 - Prob. 1.1SRCh. 17.1 - Prob. 1.2SRCh. 17.1 - Prob. 1.3SRCh. 17.1 - Prob. 1.4SRCh. 17.1 - Prob. 1.5SRCh. 17.1 - Prob. 1.6SRCh. 17.1 - Short answer: Label the kidney structures in the...Ch. 17.1 - Prob. 1.8SRCh. 17.1 - Prob. 1.9SRCh. 17.1 - Prob. 1.10SRCh. 17.1 - Prob. 1.11SRCh. 17.1 - Prob. 1.12SRCh. 17.1 - Prob. 1.13SRCh. 17.1 - Prob. 2SRCh. 17.2 - Prob. 1LOCh. 17.2 - Prob. 2LOCh. 17.2 - Summarize the major steps involved in water...Ch. 17.2 - Compare and contrast chronic and acute renal...Ch. 17.2 - Prob. 1.1RCh. 17.2 - Prob. 1.2RCh. 17.2 - Prob. 1.3RCh. 17.2 - Prob. 2.1RCh. 17.2 - What effect does an increase in ADH levels have on...Ch. 17.2 - Prob. 2.3RCh. 17.2 - Prob. 3.1RCh. 17.2 - Prob. 3.2RCh. 17.2 - Prob. 3.3RCh. 17.2 - Prob. 4.1RCh. 17.2 - Prob. 4.2RCh. 17.2 - Prob. 4.3RCh. 17.2 - Prob. 1SRCh. 17.2 - Prob. 2.1SRCh. 17.2 - Prob. 2.2SRCh. 17.2 - Prob. 2.3SRCh. 17.2 - Prob. 2.4SRCh. 17.2 - Prob. 2.5SRCh. 17.2 - Prob. 2.6SRCh. 17.2 - Prob. 2.7SRCh. 17.2 - Prob. 2.8SRCh. 17.2 - Prob. 2.9SRCh. 17.2 - Prob. 2.10SRCh. 17.2 - List six substances that diffuse out of the blood...Ch. 17.2 - Prob. 3.2SRCh. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Prob. 1.1RCh. 17.3 - Prob. 1.2RCh. 17.3 - Prob. 1.3RCh. 17.3 - Prob. 2.1RCh. 17.3 - Prob. 2.2RCh. 17.3 - Prob. 2.3RCh. 17.3 - Matching: Match each lettered term with the most...Ch. 17.3 - Prob. 1.2SRCh. 17.3 - Prob. 1.3SRCh. 17.3 - Prob. 1.4SRCh. 17.3 - Prob. 1.5SRCh. 17.3 - Prob. 1.6SRCh. 17.3 - Prob. 1.7SRCh. 17.3 - Prob. 1.8SRCh. 17.3 - Prob. 1.9SRCh. 17.3 - Prob. 1.10SRCh. 17.3 - Prob. 1.11SRCh. 17.3 - Prob. 2SRCh. 17.3 - Short answer: List four primary signs and symptoms...Ch. 17.3 - Prob. 4SRCh. 17.4 - Prob. 1LOCh. 17.4 - Summarize the relationship between sodium and...Ch. 17.4 - Prob. 1.1RCh. 17.4 - Prob. 1.2RCh. 17.4 - Prob. 1.3RCh. 17.4 - Prob. 2.1RCh. 17.4 - Prob. 2.2RCh. 17.4 - Prob. 2.3RCh. 17.4 - Prob. 1.1SRCh. 17.4 - Prob. 1.2SRCh. 17.4 - Prob. 1.3SRCh. 17.4 - Prob. 1.4SRCh. 17.4 - Prob. 1.5SRCh. 17.4 - Prob. 1.6SRCh. 17.4 - Prob. 1.7SRCh. 17.4 - Prob. 1.8SRCh. 17.4 - Prob. 1.9SRCh. 17.4 - Prob. 1.10SRCh. 17.4 - Prob. 1.11SRCh. 17.4 - _____ Greatest contributor to the solid components...Ch. 17.4 - Prob. 1.13SRCh. 17.4 - Prob. 1.14SRCh. 17.4 - Prob. 2.1SRCh. 17.4 - Prob. 2.2SRCh. 17.4 - Prob. 2.3SRCh. 17.4 - Prob. 2.4SRCh. 17.4 - Prob. 2.5SRCh. 17.4 - Prob. 2.6SRCh. 17.4 - Prob. 3SRCh. 17.5 - Prob. 1LOCh. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.5 - Define acidemia and alkalemia.
Ch. 17.5 - Prob. 1.2RCh. 17.5 - Prob. 1.3RCh. 17.5 - Prob. 2.1RCh. 17.5 - Prob. 2.2RCh. 17.5 - Prob. 2.3RCh. 17.5 - Prob. 3.1RCh. 17.5 - Prob. 3.2RCh. 17.5 - Prob. 3.3RCh. 17.5 - Prob. 1SRCh. 17.5 - Prob. 2SRCh. 17 - Prob. 1CRQCh. 17 - Prob. 2CRQCh. 17 - Prob. 3CRQCh. 17 - Prob. 4CRQCh. 17 - Prob. 5CRQ
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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