Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 17.1, Problem 5QC
Which side of the heart is considered the systemic pump? What is the destination of blood from the systemic pump? To which side of the heart does it return?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 17 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 17.1 - Where is the heart located, and how large is it?Ch. 17.1 - What are the hearts upper and lower chambers...Ch. 17.1 - What are the sources from which blood flows into...Ch. 17.1 - 4. Which side of the heart is considered the...Ch. 17.1 - Which side of the heart is considered the systemic...Ch. 17.2 - Prob. 1QCCh. 17.2 - Prob. 2QCCh. 17.2 - 3. What are the three layers of the heart wall,...Ch. 17.2 - 4. What are the four main great vessels? From...Ch. 17.2 - How do the right and left ventricles differ in...
Ch. 17.2 - 6. Why do you think it is important to ensure via...Ch. 17.2 - 7. What is the overall pathway of blood flow...Ch. 17.2 - Prob. 8QCCh. 17.2 - Prob. 9QCCh. 17.2 - Prob. 10QCCh. 17.3 - How do pacemaker and contractile cells differ?...Ch. 17.3 - 2. What are intercalated discs? What is their...Ch. 17.3 - Prob. 3QCCh. 17.3 - Prob. 4QCCh. 17.3 - What is the sequence of events of a contractile...Ch. 17.3 - How does the refractory period of cardiac muscle...Ch. 17.3 - 7. What does an ECG record?
Ch. 17.3 - What are the five waves in an ECG, and what do...Ch. 17.4 - What causes the heart sounds S1 and S2?Ch. 17.4 - Prob. 2QCCh. 17.4 - Prob. 3QCCh. 17.4 - Is the end-diastolic or the end-systolic volume of...Ch. 17.4 - 5. Walk through the mechanical events of the...Ch. 17.4 - 6. Relate the ECG waves to the cardiac cycle...Ch. 17.4 - 7. How does the left ventricular pressure...Ch. 17.5 - Prob. 1QCCh. 17.5 - What is cardiac output? How does it relate to...Ch. 17.5 - Prob. 3QCCh. 17.5 - What is the Frank-Starling law, and how does it...Ch. 17.5 - What is a chronotropic agent?Ch. 17.5 - Prob. 6QCCh. 17.5 - 7. What effects does the parasympathetic nervous...Ch. 17.5 - How would a hormone that decreases the amount of...Ch. 17.5 - How is heart failure defined?Ch. 17 - 1. Mark the following statements as true or false....Ch. 17 - 2. The pericardial cavity is located between:
a....Ch. 17 - 3. Which of the following statements is true?
a....Ch. 17 - Match the following terms with the correct...Ch. 17 - Fill in the blanks: The coronary arteries are the...Ch. 17 - 6. How do pacemaker cardiac muscle cells differ...Ch. 17 - 7. Cardiac muscle cells are joined by structures...Ch. 17 - Prob. 8CYRCh. 17 - Prob. 9CYRCh. 17 - 10. The _________is the primary pacemaker of the...Ch. 17 - The AV node delay: a. allows the atria and...Ch. 17 - Explain what each of the following terms...Ch. 17 - 13. Mark the following statements as true or...Ch. 17 - Prob. 14CYRCh. 17 - 15. Fill in the blanks: The first heart sound is...Ch. 17 - Cardiac output is equal to: a. end-diastolic...Ch. 17 - Prob. 17CYRCh. 17 - 18. Which of the following statements is false?
a....Ch. 17 - 1. A birth defect called transposition of great...Ch. 17 - 2. Predict which would be more damaging to...Ch. 17 - 3. When the SA node doesn’t function properly, the...Ch. 17 - Prob. 4CYUCh. 17 - Prob. 1AYKACh. 17 - Prob. 2AYKACh. 17 - Prob. 3AYKACh. 17 - Prob. 4AYKB
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