BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17.1, Problem 1LO
Summary Introduction

To describe: The way in which the ligases and restriction endonucleases are used to make recombinant DNA.

Introduction: Deoxyribonucleic acid (DNA) is the hereditary material present in humans and almost all organisms. It contains the genetic information required for the development and functioning of living organisms. A single DNA molecule formed using DNA from two different sources is called as the recombinant DNA.

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b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
Explain the role of restriction endonucleases in the process of genetic engineering.
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