Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 17, Problem 9P
Interpretation Introduction

Interpretation:

Difference between the catalytic and stoichiometric coenzymes in the pyruvate dehydrogenase complex.

Concept introduction:

Pyruvate dehydrogenase enzyme complex is required for the linking of glycolysis and TCA. The five enzymes which form the pyruvate dehydrogenase complex are pyruvate dehydrogenase, dihydrolipoyl dehydrogenase, Kinase, dihydrolipoyl transacetylase, and phosphatase. It helps in the conversion of pyruvate into Acetyl CoA. Acetyl CoA then enters into the series of the citric acid cycle.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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