LSC ANATOMY & PHYSIOLOGY CONNECT ACCESS
4th Edition
ISBN: 9781264929290
Author: McKinley
Publisher: MCG CUSTOM
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Textbook Question
Chapter 17, Problem 20DYKB
Discuss the homeostatic system involving insulin.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 17 Solutions
LSC ANATOMY & PHYSIOLOGY CONNECT ACCESS
Ch. 17.1 - Prob. 1WDYLCh. 17.1 - Prob. 2WDYLCh. 17.1 - Prob. 3WDYLCh. 17.2 - Prob. 4WDYLCh. 17.2 - Prob. 5WDYLCh. 17.3 - Identify which of the following hormone categories...Ch. 17.3 - What two events or processes associated with a...Ch. 17.3 - Prob. 8WDYLCh. 17.4 - Why are carrier proteins necessary for...Ch. 17.4 - What is the added benefit of a carrier protein?
Ch. 17.4 - What is the relationship of hormone synthesis to...Ch. 17.5 - Where are lipid-soluble hormone receptors located?...Ch. 17.5 - Prob. 13WDYLCh. 17.6 - How does down-regulation of cellular receptors...Ch. 17.6 - What effects are seen when hormones act...Ch. 17.7 - What is the anatomic connection between the...Ch. 17.7 - Prob. 17WDYLCh. 17.7 - Prob. 18WDYLCh. 17.7 - Prob. 19WDYLCh. 17.7 - Prob. 20WDYLCh. 17.8 - Prob. 21WDYLCh. 17.8 - What is the relationship of TRH, TSH, and TH in...Ch. 17.8 - What are the primary target organs/issues of TH?...Ch. 17.8 - Prob. 24WDYLCh. 17.9 - Prob. 25WDYLCh. 17.9 - Prob. 26WDYLCh. 17.9 - Prob. 27WDYLCh. 17.10 - Prob. 28WDYLCh. 17.10 - Prob. 29WDYLCh. 17.10 - Prob. 30WDYLCh. 17.10 - Prob. 31WDYLCh. 17.11 - Prob. 32WDYLCh. 17.11 - Prob. 33WDYLCh. 17.11 - Prob. 34WDYLCh. 17.11 - Prob. 35WDYLCh. 17.12 - Prob. 36WDYLCh. 17 - Prob. 1DYKBCh. 17 - This hormones primary function is to regulate...Ch. 17 - Which of the following are components of...Ch. 17 - A hormone released from the anterior pituitary is...Ch. 17 - Prob. 5DYKBCh. 17 - Prob. 6DYKBCh. 17 - Glucagon has an __________ effect to insulin on...Ch. 17 - Glucocorticoids (e.g., cortisol) are produced in...Ch. 17 - Thyroid-stimulating hormone stimulates the a....Ch. 17 - Prob. 10DYKBCh. 17 - Prob. 11DYKBCh. 17 - Prob. 12DYKBCh. 17 - Explain the three mechanisms used to stimulate...Ch. 17 - Identify the three chemical classes of hormones,...Ch. 17 - Describe how local hormones differ from...Ch. 17 - Explain the function of carrier proteins in...Ch. 17 - Describe how water-soluble hormones interact with...Ch. 17 - Explain how the hypothalamus oversees and controls...Ch. 17 - Explain how the hypothalamus oversees and controls...Ch. 17 - Discuss the homeostatic system involving insulin.Ch. 17 - George is a 43-year-old construction worker who...Ch. 17 - What is the best diagnostic test to determine if...Ch. 17 - Jelena is late for work and is rushing to get out...Ch. 17 - Blood samples from a young woman named Michelle...Ch. 17 - Stephen is taking a new weight-loss supplement...Ch. 17 - Prob. 1CSLCh. 17 - Prob. 2CSLCh. 17 - Henry is a well-informed patient who is interested...
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