The town with most of the rats susceptible to warfarin. Introduction: Rodenticides are the pesticides that have a capability to kill the whole rodent species. Rodents have their own importance in nature but sometimes require some control over their population, as they could transmit disease, destroy crops, and may cause ecological damage. Warfarin is a common anticoagulant that could be used as a rodenticide to kill mice and rats. After the discovery, warfarin has been widely used and later it resulted in the development of species of warfarin-resistant mice and rats.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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Chapter 17, Problem 1DAA

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To determine: The town with most of the rats susceptible to warfarin.

Introduction: Rodenticides are the pesticides that have a capability to kill the whole rodent species. Rodents have their own importance in nature but sometimes require some control over their population, as they could transmit disease, destroy crops, and may cause ecological damage. Warfarin is a common anticoagulant that could be used as a rodenticide to kill mice and rats. After the discovery, warfarin has been widely used and later it resulted in the development of species of warfarin-resistant mice and rats.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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