FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 17, Problem 15E
Summary Introduction
To calculate: The ratio of [fumarate] to [succinate] under cellular conditions at 37°C.
Concept introduction: The physiological free energy changes of a reaction determine its fate in the
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Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa).
Lane 1: buffer
Lane 2 : Ladder
Lane 3: Lysate
Lane 4: Flowthrough
Lane 5: Wash
Lanes 6-8: E1, E2, E3
Lane 9: Dialyzed VRK1
Lane 10: LDH
Please help
You have isolated a protein and determined that the native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
Describe the architecture of the polypeptide component of this enzyme.
Chapter 17 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 17 - Prob. 1ECh. 17 - 2. If acetyl-CoA is labeled with l4C at its methyl...Ch. 17 - 3. List four possible metabolic fates for pyruvate...Ch. 17 - 4. Explain why metabolic acidosis (Box 2-2) may...Ch. 17 - 5. Which one of the live steps of the pyruvate...Ch. 17 - Prob. 6ECh. 17 - 7. Photosynthetic organisms use elaborate...Ch. 17 - 6. Does the pyruvate-formate lyase reaction...Ch. 17 - Prob. 9ECh. 17 - Prob. 10E
Ch. 17 - Prob. 11ECh. 17 - 12. What citric acid cycle intermediates would...Ch. 17 - Prob. 13ECh. 17 - Prob. 14ECh. 17 - Prob. 15ECh. 17 - 16. What is the ratio of [isocitrate] to [citrate]...Ch. 17 - 17. Explain why a deficiency of pyruvate...Ch. 17 - Prob. 18ECh. 17 - Prob. 19ECh. 17 - Prob. 20ECh. 17 - 21. Helicobacter pylori, which causes gastric...Ch. 17 - Prob. 22ECh. 17 - Prob. 23CQCh. 17 - 24. Which compounds would accumulate in an...Ch. 17 - Prob. 25CQCh. 17 - 26. Certain microorganisms with an incomplete...Ch. 17 - Prob. 27CQCh. 17 - Prob. 28CQCh. 17 - Prob. 29CQCh. 17 - Prob. 30CQCh. 17 - 31. Anaplerotic reactions permit the citric acid...Ch. 17 - 32. Although animals cannot synthesize glucose...Ch. 17 - Prob. 33CQCh. 17 - Prob. 34CQCh. 17 - Prob. 35CQCh. 17 - 36. Acetyl-CoA, in addition to providing 2-carbon...Ch. 17 - Prob. 1MTE
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