The reaction involved in the conversion of isocitrate into α- ketoglutarate. Introduction: Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

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Answer 1

Question

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

Expert Solution & Answer

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Answer 3

Question

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

Answer 6

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Answer 7

Chapter 16, Problem 8P

Summary Introduction

To determine: The reaction involved in the conversion of isocitrate into α- ketoglutarate.

Introduction:

Citric acid cycle is a chain of redox reactions to produce some amount of energy. Citric acid cycle takes place in multiple steps; the third step of the cycle is the conversion of isocitrate into α- ketoglutarate. Oxidative decarboxylation of the isocitrate takes place which produces α- ketoglutarate.

Answer 8

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Answer 9

Summary Introduction

To determine: The role of cofactor in the chemical reaction of conversion of isocitrate into α- ketoglutarate.

Introduction:

Every enzyme present in the cell catalyzes the reactions of the cell. There is a non protein part that is associated with the enzyme, which is called a cofactor or prosthetic group. These cofactors are responsible for the proper functioning of the enzymes. Enzyme cannot perform in the absence of cofactors.

Answer 10

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

Answer 11

Summary Introduction

To determine: Similar reactions of citric acid cycle with the conversion of isocitrate into α- ketoglutarate.

Introduction:

Conversion of isocitrate into α- ketoglutarate is a decarboxylation process and it occurs in the presence of the enzyme isocitrate dehydrogenase. This enzyme reduces isocitrate to yield α- ketoglutarate. This reaction is the third step of the citric acid cycle, the similar reactions may take place in the whole cycle.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 16 - Prob. 1P Ch. 16 - Prob. 2P Ch. 16 - Prob. 3P Ch. 16 - Prob. 4P Ch. 16 - Prob. 5P Ch. 16 - Prob. 6P Ch. 16 - Prob. 7P Ch. 16 - Prob. 8P Ch. 16 - Prob. 9P Ch. 16 - Prob. 10P

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