The process that can be performed to distinguish the genes involved in general stress response and the ones specific for osmolarity change. Introduction: One culture of E.coli is grown in a medium with high osmolarity, and another culture of E.coli is grown in a medium with low osmolarity to find the genes that are turned on or off in response to changes in osmolarity. RNA-seq analysis was performed for each culture. It was then hypothesized that osmotic changes might induce a general stress response that may be seen with other stressors like heat shock.

Question

Want to see the full answer?

Answer 1

Question

Chapter 16, Problem 37P

Summary Introduction

To determine:

The process that can be performed to distinguish the genes involved in general stress response and the ones specific for osmolarity change.

Introduction:

One culture of E.coli is grown in a medium with high osmolarity, and another culture of E.coli is grown in a medium with low osmolarity to find the genes that are turned on or off in response to changes in osmolarity. RNA-seq analysis was performed for each culture. It was then hypothesized that osmotic changes might induce a general stress response that may be seen with other stressors like heat shock.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:

a. What percentage of a drug is eliminated after 4 half-lives? Please round to the nearest percent. b. What will happen to elimination of the drug in the previous question if the system is saturated? explain and show any math involved