
EBK BIOCHEMISTRY
6th Edition
ISBN: 9781337431200
Author: GRISHAM
Publisher: CENGAGE LEARNING - CONSIGNMENT
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Chapter 16, Problem 2P
Interpretation Introduction
To determine:
At which step in the contraction cycle an ATP analog ß,?-methylene-ATP is capable of inhibiting muscle contraction.
Introduction:
Muscle contraction is a cyclic process in which actin and myosin proteins undergo conformational changes with the help of ATP. In the beginning of the cycle myosin head is energized and has bound ADP molecule and an inorganic phosphate group.
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Consider the reaction.
kp
S P
kg
What effects are produced by an enzyme on the general reaction?
AG for the reaction increases.
The rate constant for the reverse reaction (kr) increases.
The reaction equilibrium is shifted toward the products.
The concentration of the reactants is increased.
The activation energy for the reaction is lowered.
The formation of the transition state is promoted.
The graph displays the activities of wild-type and several mutated forms of subtilisin on a logarithmic scale.
The mutations are identified as:
• The first letter is the one-letter
abbreviation for the amino acid
being altered.
• The number identifies the
position of the residue in the
primary structure.
⚫ The second letter is the
one-letter abbreviation for the
amino acid replacing the
original one.
• Uncat. refers to the estimated
rate for the
uncatalyzed reaction.
Log₁(S-1)
Wild type
S221A
H64A
-5
D32A
S221A
H64A
D32A
-10
Uncat.
How would the activity of a reaction catalyzed by a version of subtilisin with all three residues in the catalytic triad
mutated compare to the activity of the uncatalyzed reaction?
It would have more activity, because the reaction catalyzed by the triple mutant is approximately three-fold faster
than the uncatalyzed reaction.
It would have less activity, because the reaction catalyzed by the triple mutant is approximately 1000-fold slower
than the…
B
Substrate
Product
AL
Product
Substrate
Reaction progress-
Reaction progress-
omplete the passage describing the two reactions.
In reaction A, the stability of the substrate is
(AG) of the reaction is
positive,
Incorrect Answer
greater than the stability of the product. The free-energy change
Incorrect Answer
so the reaction is considered
In reaction B, the stability of the substrate is
(AG) of the reaction is
less than
Incorrect Answer
endergonic
and
Incorrect Answer
not spontaneous.
Incorrect Answer
the stability of the product. The free-energy change
negative, so the reaction is considered
Incorrect Answer
exergonic
and
spontaneous.
Incorrect Answer
Incorrect Answer
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