Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 16, Problem 20P
Summary Introduction

To analyze:

The majority of organisms exhibit a circadian rhythm, a cycling of biological processes that are generally harmonized with day length (e.g., jet lag arises in humans when rapid movement between time zones causes established circadian rhythms to be out of synch with daylight hours). In Drosophila, pupae eclose (appear as adults after metamorphosis) at the time of dawn. Using this information,

Outline the screening method for Drosophila mutants that have an impaired circadian rhythm.

In majority of plants, such as Arabidopsis, genes whose encoded products have roles linked to photosynthesis have expression patterns that differ in a circadian manner. Using this information, outline the screening method for Arabidopsis mutants that have an impaired circadian rhythm.

Analyze the method for cloning of the genes that are identified by mutation.

Introduction:

A circadian rhythm is a natural process that is present in most living organisms. It is a biological process that regulates the wake-sleep cycle and repeats the cycle every 24 hours. This 24 hour cycle is driven by a circadian clock. The study of circadian clock is known as chronobiology. It is widely seen in animals, fungi, plants, and cyanobacteria. Circadian rhythms are endogenous but are adjusted to the local environment by light and temperature.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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