Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 16, Problem 18P
In land plants, there is an alternation of generations between a haploid gametophyte generation and a diploid sporophytic generation. Both generations are typically multicellular and may be free
How would you conduct a screen to identify genes required for female gametophyte development in Arabidopsis?
How would you conduct a screen to identify genes required for male gametophyte development?
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In the absence of centrioles among the cells of higher plants, give one pole-based mechanism exhibited by such cells and briefly explain how it helps chromosomes be pulled in opposite poles.
Alternation of generations means
-One phase of the life cycle is photosynthetic and the other is heterotrophic.
-One phase of the life cycle is unicellular and the other is multicellular.
-One phase of the life cycle takes place on land and the other in water.
-One phase of the life cycle is multicellular diploid and the other is multicellular haploid.
-One phase of the life cycle is motile and the other is stationary.
The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid:
a. Between which two stages of the Neurospora life cycle do most mitotic cell divisions occur?
b. Neurospora has an arginine amino acid synthesis pathway shown below:
Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning.
c. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain:
i) What percentage of the progeny will grow on ornithine?
ii) What percentage on citrulline?
iii) What percentage on arginine?
Show your work for i), ii) and iii). [Can be answered in less…
Chapter 16 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 16 - 14.1 What are the advantages and disadvantages of...Ch. 16 - Prob. 2PCh. 16 - 3. Genetic maps and physical maps are both...Ch. 16 - 14.5 What are the advantages and disadvantages of...Ch. 16 - 14.6 You have cloned the mouse ortholog (see...Ch. 16 - 14.13 The CBF genes of Arabidopsis are induced by...Ch. 16 - 14.14 When the S. cerevisiae genome was sequenced,...Ch. 16 - 14.15 Translational fusions between a protein of...Ch. 16 - In enhancer trapping experiments, a minimal...Ch. 16 - 14.19 In Genetic Analysis, we designed a screen to...
Ch. 16 - How would you design a genetic screen to find...Ch. 16 - 14.21 The eyes of Drosophila develop from imaginal...Ch. 16 - 14.22 Given your knowledge of the genetic tools...Ch. 16 - Mutations in the CFTR gene result in cystic...Ch. 16 - Prob. 16PCh. 16 - 14.25 How would you conduct a screen to identify...Ch. 16 - In land plants, there is an alternation of...Ch. 16 - 14.27 The Drosophila evenskipped (eve) gene is...Ch. 16 - Prob. 20PCh. 16 - 14.29 As shown in Figure, mutations in the...
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