The phenotype of a repressor mutation which prevents DNA binding. Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

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Answer 1

Question

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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Answer 3

Question

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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Answer 4

Question

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

Answer 6

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Answer 7

Chapter 16, Problem 1DA

Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

Answer 8

Expert Solution

Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Answer 9

Expert Solution

Answer 10

Expert Solution

Answer 11

Expert Solution

Answer 12

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

Answer 13

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Answer 14

Expert Solution

Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.