Biology
Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 16, Problem 1DA
Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

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Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

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Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY