Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 16, Problem 16P
For each of the following mutant E. coli strains, plot a 30-minute time course of concentration of β-galactosidase, permease, and acetylase enzymes grown under the following conditions: For the first 10 minutes, no lactose is present; at 10 minutes, lactose becomes the sole carbon source. Plot concentration on the y-axis, time on the x-axis. (Don’t worry about the exact units for each protein on the y-axis.)
| a. | I-P+o+Z+Y+A+/ I+P+o+Z-Y+A+ |
| b. | I-P+ocZ+Y+A+/ I+P+o+Z-Y+A+ |
| c. | IsP+o+Z+Y+A+/ I-P+o+Z-Y+A+ |
| d. | I-P-o+Z+Y+A+/ I-P+ocZ+Y-A+ |
| e. | I-P+o+Z-Y+A+/ I-P-ocZ+Y-A+ |
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Students have asked these similar questions
For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of β-galactosidase and permease and a minus sign (–) to indicate no synthesis of the proteins.
For each of the following genotypes, explain how mutation (identified by a (-) will affect the
organism grown in lactose medium. Indicate whether
a) B-galactosidase will be synthesized or not,
b) synthesis of B-galactosidase is inducible (1) or constitutive (C) and
c) growth of the organism will occur or not.
i. I+P+0-Z+Y+A+/l+P+O+Z-Y-A-
ii. I-P+0+Z+Y+A+/I+P-O+Z+Y+A+
Clary Leonhart used the pET vector system to express her prokaryotic amylase enzyme. She
added peptone into her culture broth of BL21 (DE3) Escherichia coli strain. At the end of the
experiment, she discovered that her protein was not expressed. She repeated three more times
but her protein of interest was still not produced.
(i)
(ii)
Explain the reason why Clary failed to obtain her protein of interest and suggest a
solution to troubleshoot this problem.
Clary plans to express her protein along with a polyhistidine-tag, or better known by
its trademarked name IIis-tag. Explain the importance of His-tag in protein work.
Chapter 16 Solutions
Genetics: From Genes to Genomes
Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - The following statement occurs early in this...Ch. 16 - One of the main lessons of this chapter is that...Ch. 16 - All mutations that abolish function of the Rho...Ch. 16 - The figure at the beginning of this chapter shows...Ch. 16 - The promoter of an operon is the site to which RNA...Ch. 16 - You are studying an operon containing three genes...Ch. 16 - You have isolated a protein that binds to DNA in...Ch. 16 - You have isolated two different mutants reg1 and...Ch. 16 - Bacteriophage , after infecting a cell, can...
Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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