Biological Science (6th Edition)
Biological Science (6th Edition)
6th Edition
ISBN: 9780321976499
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Emily Taylor, Greg Podgorski, Jeff Carmichael
Publisher: PEARSON
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Chapter 16, Problem 14PIAT
Summary Introduction

To review:

Whether the mRNA (messenger ribonucleic acid) produced by the zebra fish, puffer fish, mice, and humans have same mRNA base-pair sequence or not.

Introduction:

The eukaryotic mRNA is defined as the molecule that determines the amino acid sequence of the protein. The structure of the eukaryotic mRNA includes the five-prime cap (5′ cap), 5’ UTR (untranslated region), coding sequence, 3’ UTR, and the poly (A) (poly-adenine monophosphates) tail.

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Amino Acid Coclow TABle 3' Gly Phe Leu (G) (F) (L) 3- Val (V) Arg (R) Ser (S) Ala (A) Lys (K) CAG G Glu Asp (E) (D) Ser (S) CCCAGUCAGUCAGUCAG 0204 C U A G C Asn (N) G 4 A AGU C GU (5) AC C UGA A G5 C CUGACUGACUGACUGAC Thr (T) Met (M) lle £€ (1) U 4 G Tyr Σε (Y) U Cys (C) C A G Trp (W) 3' U C A Leu בוט His Pro (P) ££ (H) Gin (Q) Arg 흐름 (R) (L) Start Stop 8. Transcription and Translation Practice: (Video 10-1 and 10-2) A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense DNA strand and label the 5' and 3' ends. B. Use the antisense strand that you create in part A as a template to create the mRNA transcript of the gene and label the 5' and 3' ends. C. Translate the mRNA you produced in part B into the polypeptide sequence making sure to follow all the rules of translation. 5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand) 4
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