Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 15, Problem 6EQ
As described in Chapter 21, an electrophoretic mobility shift assay (EMSA) can be used to determine if a protein binds to a segment of DNA. When a segment of DNA is bound by a protein, its mobility will be retarded, and the DNA band will appear higher in the gel. In an EMSA whose results are shown below, a cloned gene fragment that is
in length contains a regulatory element that is recognized by a transcription factor called protein X. Previous experiments have shown that the presence of hormone X results in transcriptional activation by protein X.
Explain the action of hormone X.
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Chapter 15 Solutions
Genetics: Analysis and Principles
Ch. 15.1 - 1. Combinatorial control refers to the phenomenon...Ch. 15.1 - 2. A regulatory transcription factor protein...Ch. 15.1 - 3. A bidirectional enhancer has the following...Ch. 15.1 - 4. Regulatory transcription factors can be...Ch. 15.2 - 1. A chromatin-remodeling complex may
a. change...Ch. 15.2 - Prob. 2COMQCh. 15.2 - 3. Which of the following characteristics is...Ch. 15.2 - 4. Transcriptional activation of eukaryotic genes...Ch. 15.3 - How can methylation affect transcription? a. It...Ch. 15.3 - 2. The process in which completely unmethylated...
Ch. 15.4 - Prob. 1COMQCh. 15.5 - The overall goal of the ENCODE Project is a. to...Ch. 15 - Discuss the common points of control in eukaryotic...Ch. 15 - 2. Discuss the structure and function of...Ch. 15 - 3. What is meant by the term transcription factor...Ch. 15 - What are the functions of transcriptional...Ch. 15 - 5. Is each of the following statements true or...Ch. 15 - 6. Transcription factors usually contain one or...Ch. 15 - Prob. 7CONQCh. 15 - Prob. 8CONQCh. 15 - 9. Let’s suppose a mutation in the glucocorticoid...Ch. 15 - Prob. 10CONQCh. 15 - Prob. 11CONQCh. 15 - Prob. 12CONQCh. 15 - 13. Transcription factors such as the...Ch. 15 - An enhancer, located upstream from a gene, has the...Ch. 15 - 15. The DNA-binding domain of each CREB protein...Ch. 15 - The gene that encodes the enzyme called tyrosine...Ch. 15 - Prob. 16CONQCh. 15 - Prob. 18CONQCh. 15 - 18. What is a histone variant?
Ch. 15 - Prob. 20CONQCh. 15 - 20. What is meant by the term histone code? With...Ch. 15 - Prob. 22CONQCh. 15 - Histones are thought to be displaced as RNA...Ch. 15 - 23. What is an insulator? Describe two different...Ch. 15 - 24. What is DNA methylation? When we say that DNA...Ch. 15 - Lets suppose that a vertebrate organism carries a...Ch. 15 - 26. What is a CpG island? Where would you expect...Ch. 15 - 1. Briefly describe the method of chromatin...Ch. 15 - Researchers can isolate a sample of cells, such as...Ch. 15 - Prob. 3EQCh. 15 - Prob. 4EQCh. 15 - Prob. 5EQCh. 15 - 6. As described in Chapter 21, an electrophoretic...Ch. 15 - 1. Explain how DNA methylation could be used to...Ch. 15 - 2. Enhancers can occur almost anywhere in DNA and...
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- 10. Your instructor will give you 2 amino acids during the activity session (video 2-7. A. First color all the polar and non-polar covalent bonds in the R groups of your 2 amino acids using the same colors as in #7. Do not color the bonds in the backbone of each amino acid. B. Next, color where all the hydrogen bonds, hydrophobic interactions and ionic bonds could occur in the R group of each amino acid. Use the same colors as in #7. Do not color the bonds in the backbone of each amino acid. C. Position the two amino acids on the page below in an orientation where the two R groups could bond together. Once you are satisfied, staple or tape the amino acids in place and label the bond that you formed between the two R groups. - Polar covalent Bond - Red - Non polar Covalent boND- yellow - Ionic BonD - PINK Hydrogen Bonn - Purple Hydrophobic interaction-green O=C-N H I. H HO H =O CH2 C-C-N HICK H HO H CH2 OH H₂N C = Oarrow_forwardFind the dental formula and enter it in the following format: I3/3 C1/1 P4/4 M2/3 = 42 (this is not the correct number, just the correct format) Please be aware: the upper jaw is intact (all teeth are present). The bottom jaw/mandible is not intact. The front teeth should include 6 total rectangular teeth (3 on each side) and 2 total large triangular teeth (1 on each side).arrow_forward12. Calculate the area of a circle which has a radius of 1200 μm. Give your answer in mm² in scientific notation with the correct number of significant figures.arrow_forward
- Describe the image quality of the B.megaterium at 1000X before adding oil? What does adding oil do to the quality of the image?arrow_forwardWhich of the follwowing cells from this lab do you expect to have a nucleus and why or why not? Ceratium, Bacillus megaterium and Cheek epithelial cells?arrow_forward14. If you determine there to be debris on your ocular lens, explain what is the best way to clean it off without damaging the lens?arrow_forward
- 11. Write a simple formula for converting mm to μm when the number of mm's is known. Use the variable X to represent the number of mm's in your formula.arrow_forward13. When a smear containing cells is dried, the cells shrink due to the loss of water. What technique could you use to visualize and measure living cells without heat-fixing them? Hint: you did this technique in part I.arrow_forward10. Write a simple formula for converting μm to mm when the number of μm's are known. Use the variable X to represent the number of um's in your formula.arrow_forward
- 8. How many μm² is in one cm²; express the result in scientific notation. Show your calculations. 1 cm = 10 mm; 1 mm = 1000 μmarrow_forwardFind the dental formula and enter it in the following format: I3/3 C1/1 P4/4 M2/3 = 42 (this is not the correct number, just the correct format) Please be aware: the upper jaw is intact (all teeth are present). The bottom jaw/mandible is not intact. The front teeth should include 6 total rectangular teeth (3 on each side) and 2 total large triangular teeth (1 on each side).arrow_forwardAnswer iarrow_forward
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