Concept explainers
Which of the following is not a property of DNA polymerase?
a. It adds dNTPs only in the 5' → 3' direction.
b. It requires a primer to work.
c. It is associated with a sliding clamp only on the leading strand.
d. Its exonuclease activity is involved in proofreading.
Introduction:
DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.
Answer to Problem 1TYK
Correct answer:
The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.
Explanation of Solution
Explanation/Justification for the correct answer:
Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.
Explanation for incorrect answers:
Option (a) is given as adding of dNTPs only in the
Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.
Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.
Hence, the options (a), (b), and (d) are incorrect.
DNA polymerase catalyzes the addition of dNTPs in the
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Chapter 15 Solutions
Biological Science (7th Edition)
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- Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A. Yes, because the 3' OH is already present in the DNA strand which will be used as a template. B. No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. C. Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. D. No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first.arrow_forwardThere are several factors that contribute to high fidelity of DNA replication. Which of the following statements is INCORRECT? DNA polymerase I has a 3'-5' exonuclease editing activity. O b. RNA primers are used instead of DNA primers. There are balanced levels of dNTPs inside the cell. O C. O d. DNA polymerase III has a 3'-5' exonuclease editing activity. O e. Base-pair geometry plays important role for maintaining the high fidelity of DNA synthesis.arrow_forwardThe diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.arrow_forward
- DNA synthesis has a very low error rate. One reason for this is that the DNA polymerase enzyme can verify “in the moment” that the nucleotide it is adding to the chain is the correct complementary base before moving on. This process is called Select one: a. mismatch repair b. transcription c. proofreading d. ligationarrow_forwardWhich of the following statements is FALSE regarding the molecular mechanism for DNA polymerases? A. The active site contains 2 divalent metal ions B. A single stranded DNA template is required C. The enzyme can only attach a new deoxynucleotide to the 5’ end of a growing chain D. The 3’OH on the deoyxyribose ring attacks a phosphate of a dNTP to produce a new phosophodiester bond E. None of the above (all are true statements)arrow_forwardOne common feature of all DNA polymerases is that they a. synthesize DNA in the 3′-to-5′ direction. b. synthesize DNA in the 5′-to-3′ direction. c. synthesize DNA in both directions by switching strands. d. do not require a primer.arrow_forward
- a. unwinds the DNA helix b. stabilizes and stops the two strands from annealing (rebinding with each other) c. recoils the DNA d. cleaves both strands of DNA to relieve tension at supercoils e. places RNA primers at their proper location on the template strands f. acts as starting points for DNA polymerase g. adds DNA nucleotides to form new DNA strands h. forms phosphodiester bonds to join Okazaki fragments Esc 1. single-strand binding protein 2. helicase 3. DNA ligase 4. RNA primer 5. gyrase 6. DNA polymerase 53°F Cloudy 3 Q Search $ F5 FRarrow_forwardHow would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymerasearrow_forwardIn the following drawing, the top strand is the template DNA, and the bottom strand shows the lagging strand prior to the action of DNA polymerase I. The lagging strand contains three Okazaki fragments. The RNA primers have not yet been removed. A. Which Okazaki fragment was made first, the one on the left or the one on the right? B. Which RNA primer will be the first one to be removed by DNA polymerase I, the primer on the left or the primer on the right? For this primer to be removed by DNA polymerase I and for the gap to be filled in, is it necessary for the Okazaki fragment in the middle to have already been synthesized? Explain. C. Let’s consider how DNA ligase connects the left Okazaki fragment with the middle Okazaki fragment. After DNA polymerase I removes the middle RNA primer and fills in the gap with DNA, where does DNA ligase function? See the arrows on either side of the middle RNA primer. Is ligase needed at the left arrow, at the right arrow, or both?arrow_forward
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