Concept explainers
Which of the following is not a property of DNA polymerase?
a. It adds dNTPs only in the 5' → 3' direction.
b. It requires a primer to work.
c. It is associated with a sliding clamp only on the leading strand.
d. Its exonuclease activity is involved in proofreading.
Introduction:
DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.
Answer to Problem 1TYK
Correct answer:
The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.
Explanation of Solution
Explanation/Justification for the correct answer:
Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.
Explanation for incorrect answers:
Option (a) is given as adding of dNTPs only in the
Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.
Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.
Hence, the options (a), (b), and (d) are incorrect.
DNA polymerase catalyzes the addition of dNTPs in the
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Chapter 15 Solutions
Biological Science (7th Edition)
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- A cloned fragment of DNA was sequenced by using thedideoxy chain-termination method. A part of the autoradiogram of the sequencing gel is represented here.ddA ddG ddT ddCa. Deduce the nucleotide sequence of the DNAnucleotide chain synthesized from the primer. Label the5′ and 3′ ends.b. Deduce the nucleotide sequence of the DNAnucleotide chain used as the template strand. Label the5′ and 3′ ends.c. Write out the nucleotide sequence of the DNA doublehelix (label the 5′ and 3′ ends)arrow_forwardHow would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymerasearrow_forwardIn the following drawing, the top strand is the template DNA, and the bottom strand shows the lagging strand prior to the action of DNA polymerase I. The lagging strand contains three Okazaki fragments. The RNA primers have not yet been removed. A. Which Okazaki fragment was made first, the one on the left or the one on the right? B. Which RNA primer will be the first one to be removed by DNA polymerase I, the primer on the left or the primer on the right? For this primer to be removed by DNA polymerase I and for the gap to be filled in, is it necessary for the Okazaki fragment in the middle to have already been synthesized? Explain. C. Let’s consider how DNA ligase connects the left Okazaki fragment with the middle Okazaki fragment. After DNA polymerase I removes the middle RNA primer and fills in the gap with DNA, where does DNA ligase function? See the arrows on either side of the middle RNA primer. Is ligase needed at the left arrow, at the right arrow, or both?arrow_forward
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