Which of the following is not a property of DNA polymerase? a. It adds dNTPs only in the 5' → 3' direction. b. It requires a primer to work. c. It is associated with a sliding clamp only on the leading strand. d. Its exonuclease activity is involved in proofreading.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look?A. The sequence will be readable for the first 20 bases only.B. The detector will detect two nucleotides at about 75% of the positions.C. The sequence will be readable for the last 20 bases only.D. The detector will detect one nucleotide at about 75% of the positions.E. The detector will detect no nucleotides at any of the positions. Explain why it is B.

Which statement about Okazaki fragments is true? Select one: a. DNA polymerase doesn’t need a primer to build these fragments b. They act as a primer that initiates DNA replication. c. They correct errors made during earlier phases of DNA replication. d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork. e. They prevent the ends of chromosomes from shortening with every replication.

Certain restriction endonucleases produce cohesive (sticky) ends. This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.

Answer 2

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Answer 8

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer 12

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Answer 13

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Answer 14

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Answer 15

Ch. 15 - 1. Which of the following is not a property of DNA...Ch. 15 - 2. What is the function of primase? a. synthesis...Ch. 15 - 3. How are Okazaki fragments synthesized? a. using...Ch. 15 - Prob. 4TYK Ch. 15 - PROCESS OF SCIENCE Researchers design experiments...Ch. 15 - 7. How does telomerase prevent linear chromosomes...Ch. 15 - Prob. 8TYU Ch. 15 - Prob. 11PIAT Ch. 15 - Prob. 12PIAT Ch. 15 - Prob. 14PIAT

Which of the following is not a property of DNA polymerase? a. It adds dNTPs only in the 5' → 3' direction. b. It requires a primer to work. c. It is associated with a sliding clamp only on the leading strand. d. Its exonuclease activity is involved in proofreading.

Concept explainers

Videos

Answer to Problem 1TYK

Explanation of Solution

Want to see more full solutions like this?

Chapter 15 Solutions