GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 15, Problem 18P
The consequences of four deletions from the region upstream of the yeast gene DBM
Which mutations(s) affect an enhancer sequence? Explain your reasoning.
Which mutation(s) affect a silencer sequence? Explainyour reasoning.
Which mutation(s) affect the promoter? Explain yourreasoning.
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Describe how transcription would be affected in the Galactose metabolizing pathway in Yeast in the presence of the following mutations.
1. A mutation that resulted in an inability of Gal80 to enter the nucleus.
2. A mutation that resulted in a lack of ability of Gal3 to bind galactose.
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region of gene X, which determines the length of the tail in mice, is mutated so
that transcription factors bind it at a much higher affinity compared to the wild-type sequence.
What is the most likely phenotypic outcome?
Tail length will not change because the enhancer is a non-coding sequence
Tail length will increased due to increased activity of the gene's promoter
Tail length will decreased because any mutation will cause a loss-of-function of these regulatory regions
Not just the tail will be enlarged because increased activity of the enhancer will impact many genes
a. How do bacteria increase the efficiency of gene expression? Is this possible in eukaryotes?
b. A mutation in the promoter of Gene K disrupts an enzyme binding site and results in the loss of
Gene K expression. Is this change in gene expression likely happening at the transcriptional or the
translational level? Explain.
c. Propose three different mutations to prevent initiation, elongation, and termination of bacterial
transcription, respectively. Explain how/why each mutation would prevent its respective step. (Hint:
mutations can be in genes that encode proteins or regulatory DNA sequences)
Chapter 15 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 15 - 13.1 Devoting a few sentences to each, describes...Ch. 15 - 13.2 Describe and give an example (real or...Ch. 15 - What is meant by the term chromatin remodeling?...Ch. 15 - 13.4 What general role does acetylation of histone...Ch. 15 - 13.5 Describe the roles of writers, readers, and...Ch. 15 - Outline the roles of RNA in eukaryotic gene...Ch. 15 - 13.7 What are the roles of the Polycomb and...Ch. 15 - Most biologists argue that the regulation of gene...Ch. 15 - Compare and contrast the transcriptional...Ch. 15 - The term heterochromatin refers to heavily...
Ch. 15 - 13.11 Compare and contrast promoters and enhancers...Ch. 15 - 13.12 What are the different chromatin...Ch. 15 - 13.13 Define epigenetics, and provide examples...Ch. 15 - What is one proposed role for lncRNAs?Ch. 15 - 13.17 A hereditary disease is inherited as an...Ch. 15 - Prob. 16PCh. 15 - A gene expressed in long muscle of the mouse is...Ch. 15 - The consequences of four deletions from the region...Ch. 15 - Provide a description of the mechanistic roles of...Ch. 15 - 13.20 A muscle enzyme called ME is produced by...Ch. 15 - 21. A muscle protein in mouse is produced through...
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