Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Question
Chapter 15, Problem 15.9QAP
Interpretation Introduction
Interpretation:
The mass in milligrams of quinine in the tablet should be determined.
Concept introduction:
The amount of quinine is the product of concentration of quinine and the ratio of the fluorescent intensity of the sample and the fluorescent intensity of the dilute sample.
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Quinine in a 1.553-g antimalarial tablet was dissolved in sufficient 0.10 M HCl to give 250 mL of solution.A 10.00-mL aliquot was then diluted to 50.00 mL with the acid. The fluorescence intensity for the dilutedsample at 347.5 nm provided a reading of 196 on an arbitrary scale. A standard 100-ppm quinine solutionregistered 125 when measured under conditions identical to those for the diluted sample. Calculate the massin milligrams of quinine in the tablet.
Exactly 5.00 mL aliquots of a
solution containing analyte X were
transferred into 50.00-mL
volumetric flasks and the pH of
the solution is adjusted to 9.0. The
following volumes of a standard
solution containing 2.00 µg/mL of
X were then added into each flask
and the mixture was diluted to
volume: 0.000, 0.500, 1.00, 1.50
and 2.00 mL. The fluorescence of
each of these solutions was
measured with a fluorometer, and
the following values were
obtained: 3.26, 4.80, 6.42, 8.02
and 9.56, respectively.
ii. Using relevant functions in
Excel, derive a
least-squares equation for
the data, and use the
parameters of this
equation to find the
concentration of the
phenobarbital in the
unknown solution.
To determine the content of Pb in a canned sausage, 5.00 g were weighed and after adequate treatment they were gauged to a volume of 100 mL, from there an aliquot of 2 mL was taken and brought to a volume of 250 mL. The readings of a series and of the sample were taken in an Atomic Absorption spectrophotometer, recording the following data:
mg Pb/mL
0
1
2
3
4
Sample
Absorbance
0.00
0.15
0.29
0.44
0.58
0.38
What is the% Pb in canned sausage?
Chapter 15 Solutions
Principles of Instrumental Analysis
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