Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 14TYR
Summary Introduction
To fill:
The correct term in the given blank.
Introduction:
The limbic system is themost important center of emotion and learningin the brain. It is basically a set of brain structures. It consists of some structures such as amygdala, hippocampus, cingulate gyrus, basal ganglia, and parts of the thalamus.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 15 Solutions
Human Anatomy
Ch. 15.1 - Prob. 1AWYKCh. 15.1 - List the three major parts of the brain and...Ch. 15.1 - Define gyrus and suicus.Ch. 15.1 - Prob. 3BYGOCh. 15.1 - Prob. 4BYGOCh. 15.1 - Prob. 5BYGOCh. 15.1 - Prob. 6BYGOCh. 15.1 - Name the two components of the brain barrier...Ch. 15.2 - Prob. 8BYGOCh. 15.2 - Prob. 9BYGO
Ch. 15.2 - Prob. 10BYGOCh. 15.2 - Describe the reticular formation and list several...Ch. 15.2 - Describe the general functions of the cerebellum.Ch. 15.3 - Prob. 1AWYKCh. 15.3 - Prob. 13BYGOCh. 15.3 - List at least six functions of the hypothalamus.Ch. 15.3 - Prob. 15BYGOCh. 15.3 - Prob. 16BYGOCh. 15.3 - Prob. 17BYGOCh. 15.3 - Prob. 18BYGOCh. 15.3 - Prob. 19BYGOCh. 15.3 - Prob. 20BYGOCh. 15.4 - Prob. 21BYGOCh. 15.4 - Prob. 22BYGOCh. 15.4 - If the oculomotor, trochlear, or abducens nerve...Ch. 15.4 - Prob. 24BYGOCh. 15.4 - Prob. 25BYGOCh. 15.5 - Prob. 26BYGOCh. 15.5 - Prob. 27BYGOCh. 15.5 - Describe the neuroanatomical and behavioral...Ch. 15 - Prob. 15.1.1AYLOCh. 15 - The meanings of rostral and caudal in CNS anatomyCh. 15 - Prob. 15.1.3AYLOCh. 15 - Prob. 15.1.4AYLOCh. 15 - Prob. 15.1.5AYLOCh. 15 - The meninges of the brain; how they differ from...Ch. 15 - Prob. 15.1.7AYLOCh. 15 - Prob. 15.1.8AYLOCh. 15 - Prob. 15.1.9AYLOCh. 15 - The location, anatomical features, and functions...Ch. 15 - Prob. 15.2.2AYLOCh. 15 - Prob. 15.2.3AYLOCh. 15 - Prob. 15.3.1AYLOCh. 15 - Prob. 15.3.2AYLOCh. 15 - Prob. 15.3.3AYLOCh. 15 - Prob. 15.3.4AYLOCh. 15 - Prob. 15.3.5AYLOCh. 15 - Prob. 15.3.6AYLOCh. 15 - Prob. 15.3.7AYLOCh. 15 - Prob. 15.3.8AYLOCh. 15 - The location, major components, and general...Ch. 15 - Prob. 15.3.10AYLOCh. 15 - Prob. 15.3.11AYLOCh. 15 - Prob. 15.3.12AYLOCh. 15 - Prob. 15.3.13AYLOCh. 15 - Prob. 15.3.14AYLOCh. 15 - Prob. 15.3.15AYLOCh. 15 - The motor functions of the basal nuclei and...Ch. 15 - Prob. 15.3.17AYLOCh. 15 - Prob. 15.3.18AYLOCh. 15 - The roles of the hypothalamus, amygdala, and...Ch. 15 - Prob. 15.3.20AYLOCh. 15 - Prob. 15.3.21AYLOCh. 15 - Prob. 15.3.22AYLOCh. 15 - Prob. 15.4.1AYLOCh. 15 - Prob. 15.4.2AYLOCh. 15 - The common effects of aging on the central nervous...Ch. 15 - Prob. 15.5.2AYLOCh. 15 - Prob. 15.5.3AYLOCh. 15 - Prob. 1TYRCh. 15 - Hearing is associated mainly with the limbic...Ch. 15 - Prob. 3TYRCh. 15 - Prob. 4TYRCh. 15 - Prob. 5TYRCh. 15 - Prob. 6TYRCh. 15 - Because of a brain lesion, a certain patient never...Ch. 15 - Prob. 8TYRCh. 15 - Prob. 9TYRCh. 15 - Prob. 10TYRCh. 15 - Prob. 11TYRCh. 15 - Prob. 12TYRCh. 15 - Prob. 13TYRCh. 15 - Prob. 14TYRCh. 15 - Prob. 15TYRCh. 15 - Prob. 16TYRCh. 15 - Your personality is determined mainly by which...Ch. 15 - Prob. 18TYRCh. 15 - Linear, analytical, and verbal thinking occurs in...Ch. 15 - Prob. 20TYRCh. 15 - Prob. 1BYMVCh. 15 - Prob. 2BYMVCh. 15 - State a meaning of each word element and give a...Ch. 15 - State a meaning of each word element and give a...Ch. 15 - Prob. 5BYMVCh. 15 - State a meaning of each word element and give a...Ch. 15 - Prob. 7BYMVCh. 15 - Prob. 8BYMVCh. 15 - Prob. 9BYMVCh. 15 - Prob. 10BYMVCh. 15 - Prob. 1TOFCh. 15 - Prob. 2TOFCh. 15 - Prob. 3TOFCh. 15 - Prob. 4TOFCh. 15 - Prob. 5TOFCh. 15 - Prob. 6TOFCh. 15 - Prob. 7TOFCh. 15 - Prob. 8TOFCh. 15 - Prob. 9TOFCh. 15 - Prob. 10TOFCh. 15 - Prob. 1TYCCh. 15 - Prob. 2TYCCh. 15 - Prob. 3TYCCh. 15 - Prob. 4TYCCh. 15 - Prob. 5TYC
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