Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 15.1.9AYLO
Summary Introduction
To write:
The constituents, significance, limitations of the brain barrier system.
Introduction:
The nervous system of a human is divided into two subdivisions: the central nervous system or CNS (includes the spinal cord and the brain) and the peripheral nervous system or PNS (includes rest all parts of the nervous system except spinal cord and brain).
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 15 Solutions
Human Anatomy
Ch. 15.1 - Prob. 1AWYKCh. 15.1 - List the three major parts of the brain and...Ch. 15.1 - Define gyrus and suicus.Ch. 15.1 - Prob. 3BYGOCh. 15.1 - Prob. 4BYGOCh. 15.1 - Prob. 5BYGOCh. 15.1 - Prob. 6BYGOCh. 15.1 - Name the two components of the brain barrier...Ch. 15.2 - Prob. 8BYGOCh. 15.2 - Prob. 9BYGO
Ch. 15.2 - Prob. 10BYGOCh. 15.2 - Describe the reticular formation and list several...Ch. 15.2 - Describe the general functions of the cerebellum.Ch. 15.3 - Prob. 1AWYKCh. 15.3 - Prob. 13BYGOCh. 15.3 - List at least six functions of the hypothalamus.Ch. 15.3 - Prob. 15BYGOCh. 15.3 - Prob. 16BYGOCh. 15.3 - Prob. 17BYGOCh. 15.3 - Prob. 18BYGOCh. 15.3 - Prob. 19BYGOCh. 15.3 - Prob. 20BYGOCh. 15.4 - Prob. 21BYGOCh. 15.4 - Prob. 22BYGOCh. 15.4 - If the oculomotor, trochlear, or abducens nerve...Ch. 15.4 - Prob. 24BYGOCh. 15.4 - Prob. 25BYGOCh. 15.5 - Prob. 26BYGOCh. 15.5 - Prob. 27BYGOCh. 15.5 - Describe the neuroanatomical and behavioral...Ch. 15 - Prob. 15.1.1AYLOCh. 15 - The meanings of rostral and caudal in CNS anatomyCh. 15 - Prob. 15.1.3AYLOCh. 15 - Prob. 15.1.4AYLOCh. 15 - Prob. 15.1.5AYLOCh. 15 - The meninges of the brain; how they differ from...Ch. 15 - Prob. 15.1.7AYLOCh. 15 - Prob. 15.1.8AYLOCh. 15 - Prob. 15.1.9AYLOCh. 15 - The location, anatomical features, and functions...Ch. 15 - Prob. 15.2.2AYLOCh. 15 - Prob. 15.2.3AYLOCh. 15 - Prob. 15.3.1AYLOCh. 15 - Prob. 15.3.2AYLOCh. 15 - Prob. 15.3.3AYLOCh. 15 - Prob. 15.3.4AYLOCh. 15 - Prob. 15.3.5AYLOCh. 15 - Prob. 15.3.6AYLOCh. 15 - Prob. 15.3.7AYLOCh. 15 - Prob. 15.3.8AYLOCh. 15 - The location, major components, and general...Ch. 15 - Prob. 15.3.10AYLOCh. 15 - Prob. 15.3.11AYLOCh. 15 - Prob. 15.3.12AYLOCh. 15 - Prob. 15.3.13AYLOCh. 15 - Prob. 15.3.14AYLOCh. 15 - Prob. 15.3.15AYLOCh. 15 - The motor functions of the basal nuclei and...Ch. 15 - Prob. 15.3.17AYLOCh. 15 - Prob. 15.3.18AYLOCh. 15 - The roles of the hypothalamus, amygdala, and...Ch. 15 - Prob. 15.3.20AYLOCh. 15 - Prob. 15.3.21AYLOCh. 15 - Prob. 15.3.22AYLOCh. 15 - Prob. 15.4.1AYLOCh. 15 - Prob. 15.4.2AYLOCh. 15 - The common effects of aging on the central nervous...Ch. 15 - Prob. 15.5.2AYLOCh. 15 - Prob. 15.5.3AYLOCh. 15 - Prob. 1TYRCh. 15 - Hearing is associated mainly with the limbic...Ch. 15 - Prob. 3TYRCh. 15 - Prob. 4TYRCh. 15 - Prob. 5TYRCh. 15 - Prob. 6TYRCh. 15 - Because of a brain lesion, a certain patient never...Ch. 15 - Prob. 8TYRCh. 15 - Prob. 9TYRCh. 15 - Prob. 10TYRCh. 15 - Prob. 11TYRCh. 15 - Prob. 12TYRCh. 15 - Prob. 13TYRCh. 15 - Prob. 14TYRCh. 15 - Prob. 15TYRCh. 15 - Prob. 16TYRCh. 15 - Your personality is determined mainly by which...Ch. 15 - Prob. 18TYRCh. 15 - Linear, analytical, and verbal thinking occurs in...Ch. 15 - Prob. 20TYRCh. 15 - Prob. 1BYMVCh. 15 - Prob. 2BYMVCh. 15 - State a meaning of each word element and give a...Ch. 15 - State a meaning of each word element and give a...Ch. 15 - Prob. 5BYMVCh. 15 - State a meaning of each word element and give a...Ch. 15 - Prob. 7BYMVCh. 15 - Prob. 8BYMVCh. 15 - Prob. 9BYMVCh. 15 - Prob. 10BYMVCh. 15 - Prob. 1TOFCh. 15 - Prob. 2TOFCh. 15 - Prob. 3TOFCh. 15 - Prob. 4TOFCh. 15 - Prob. 5TOFCh. 15 - Prob. 6TOFCh. 15 - Prob. 7TOFCh. 15 - Prob. 8TOFCh. 15 - Prob. 9TOFCh. 15 - Prob. 10TOFCh. 15 - Prob. 1TYCCh. 15 - Prob. 2TYCCh. 15 - Prob. 3TYCCh. 15 - Prob. 4TYCCh. 15 - Prob. 5TYC
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