CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
8th Edition
ISBN: 9781259880193
Author: SALADIN
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 15, Problem 13BYGO
Summary Introduction
Introduction:
The autonomic nervous system controls the involuntary reflex of the body like the heartbeat, respiration, and digestion and so on. The autonomic nervous system operates by the central nervous system which constitutes the hypothalamus, midbrain and the spinal cord.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 15 Solutions
CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
Ch. 15.1 - The fundamental function and effectors of the...Ch. 15.1 - Why this system is called autonomic; how it...Ch. 15.1 - Prob. 3AYLOCh. 15.1 - Why it cannot be said that at any given moment,...Ch. 15.1 - Basic anatomical components of the ANSCh. 15.1 - How autonomic efferent pathways differ from...Ch. 15.1 - Prob. 1BYGOCh. 15.2 - Prob. 1AYLOCh. 15.2 - Anatomy of the sympathetic chain; the number of...Ch. 15.2 - Prob. 3AYLO
Ch. 15.2 - Differences between the spinal nerve route,...Ch. 15.2 - Prob. 5AYLOCh. 15.2 - Prob. 6AYLOCh. 15.2 - The degree and significance of neural divergence...Ch. 15.2 - Why the adrenal medulla can be considered part of...Ch. 15.2 - Names and numbers of the cranial and spinal nerves...Ch. 15.2 - Prob. 10AYLOCh. 15.2 - Prob. 11AYLOCh. 15.2 - The location and functions of the enteric nervous...Ch. 15.2 - Explain why the sympathetic division is also...Ch. 15.2 - Prob. 4BYGOCh. 15.3 - Prob. 1AYLOCh. 15.3 - Prob. 2AYLOCh. 15.3 - Prob. 3AYLOCh. 15.3 - Prob. 4AYLOCh. 15.3 - Prob. 5AYLOCh. 15.3 - Prob. 6AYLOCh. 15.3 - Autonomic control of certain organs by dual...Ch. 15.3 - Prob. 8AYLOCh. 15.3 - Prob. 10BYGOCh. 15.3 - Prob. 11BYGOCh. 15.4 - Examples of the influence of the cerebral cortex,...Ch. 15 - Baro -Ch. 15 - Lyto-Ch. 15 - Prob. 3BYMVCh. 15 - Prob. 4BYMVCh. 15 - Prob. 5BYMVCh. 15 - Prob. 6BYMVCh. 15 - reno-Ch. 15 - Prob. 8BYMVCh. 15 - Prob. 9BYMVCh. 15 - Prob. 10BYMVCh. 15 - Prob. 1TYRCh. 15 - Muscarinic receptors bind a. epinephrine. b....Ch. 15 - All of the following cranial nerves except the...Ch. 15 - Which of the following cranial nerves carries...Ch. 15 - Prob. 5TYRCh. 15 - Epinephrine is secreted by a. sympathetic...Ch. 15 - Prob. 7TYRCh. 15 - The gray communicating ramus contains a. visceral...Ch. 15 - Prob. 9TYRCh. 15 - Which of these does not result from sympathetic...Ch. 15 - Certain nerve fibers are called _______ fibers...Ch. 15 - Prob. 12TYRCh. 15 - Prob. 13TYRCh. 15 - Most parasympathetic preganglionic fibers arc...Ch. 15 - Prob. 15TYRCh. 15 - Prob. 16TYRCh. 15 - Prob. 17TYRCh. 15 - Prob. 18TYRCh. 15 - Prob. 19TYRCh. 15 - Prob. 20TYRCh. 15 - Prob. 1WWTSCh. 15 - The parasympathetic nervous system controls...Ch. 15 - Voluntary control of the ANS is not possible.Ch. 15 - Prob. 4WWTSCh. 15 - Prob. 5WWTSCh. 15 - Prob. 6WWTSCh. 15 - Prob. 7WWTSCh. 15 - Prob. 8WWTSCh. 15 - Prob. 9WWTSCh. 15 - The two divisions of the ANS have cooperative...Ch. 15 - You are dicing raw onions while preparing dinner,...Ch. 15 - Prob. 2TYCCh. 15 - Prob. 3TYCCh. 15 - What would be the advantage to a wolf in having...Ch. 15 - Pediatric literature has reported many cases of...Ch. 15 - Prob. 1BYGOCh. 15 - Prob. 2BYGOCh. 15 - Prob. 3BYGOCh. 15 - Prob. 4BYGOCh. 15 - Prob. 5BYGOCh. 15 - Prob. 6BYGOCh. 15 - Prob. 7BYGOCh. 15 - Prob. 8BYGOCh. 15 - Prob. 9BYGOCh. 15 - Prob. 10BYGOCh. 15 - Prob. 11BYGOCh. 15 - Prob. 12BYGOCh. 15 - Prob. 13BYGOCh. 15 - Prob. 14BYGOCh. 15 - Prob. 15BYGO
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