Human Anatomy
6th Edition
ISBN: 9781260399820
Author: SALADIN, Kenneth
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 14.2, Problem 8BYGO
List the five plexuses of spinal nerves and state where each one is located.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 14 Solutions
Human Anatomy
Ch. 14.1 - Prob. 1AWYKCh. 14.1 - Prob. 1BYGOCh. 14.1 - Prob. 2BYGOCh. 14.1 - Prob. 3BYGOCh. 14.1 - Prob. 4BYGOCh. 14.1 - Identify each of the following spinal tracts with...Ch. 14.2 - How does the structure of a nerve compare to that...Ch. 14.2 - Prob. 6BYGOCh. 14.2 - Prob. 7BYGOCh. 14.2 - List the five plexuses of spinal nerves and state...
Ch. 14.2 - State which plexus gives rise to each of the...Ch. 14.3 - Prob. 1AWYKCh. 14.3 - Prob. 10BYGOCh. 14.3 - Prob. 11BYGOCh. 14.3 - Describe a situation in which each of the...Ch. 14.4 - Prob. 1AWYKCh. 14.4 - Prob. 13BYGOCh. 14.4 - Prob. 14BYGOCh. 14.4 - Prob. 15BYGOCh. 14 - Four functions of the spinal cord and their...Ch. 14 - Prob. 14.1.2AYLOCh. 14 - Prob. 14.1.3AYLOCh. 14 - Prob. 14.1.4AYLOCh. 14 - Prob. 14.1.5AYLOCh. 14 - Prob. 14.1.6AYLOCh. 14 - Prob. 14.1.7AYLOCh. 14 - Prob. 14.1.8AYLOCh. 14 - The funiculi and tracts of spinal white matterCh. 14 - Prob. 14.1.10AYLOCh. 14 - Prob. 14.1.11AYLOCh. 14 - Prob. 14.1.12AYLOCh. 14 - Prob. 14.1.13AYLOCh. 14 - Prob. 14.2.1AYLOCh. 14 - The differences between afferent and efferent...Ch. 14 - Prob. 14.2.3AYLOCh. 14 - Prob. 14.2.4AYLOCh. 14 - The number of spinal nerves and the system for...Ch. 14 - The structure of the proximal portion of a spinal...Ch. 14 - Prob. 14.2.7AYLOCh. 14 - The five plexuses of spinal nerves-their names,...Ch. 14 - Prob. 14.3.1AYLOCh. 14 - The components of a reflex arc and the path...Ch. 14 - Prob. 14.3.3AYLOCh. 14 - The differences between ipsilateral,...Ch. 14 - Prob. 14.3.5AYLOCh. 14 - Prob. 14.4.1AYLOCh. 14 - Prob. 14.4.2AYLOCh. 14 - Prob. 14.4.3AYLOCh. 14 - Below L2, the vertebral canal is occupied by a...Ch. 14 - The brachial plexus gives rise to all of the...Ch. 14 - Prob. 3TYRCh. 14 - Prob. 4TYRCh. 14 - Prob. 5TYRCh. 14 - Prob. 6TYRCh. 14 - In the spinal cord, the neurosomas of the lower...Ch. 14 - Prob. 8TYRCh. 14 - Prob. 9TYRCh. 14 - Prob. 10TYRCh. 14 - Prob. 11TYRCh. 14 - Prob. 12TYRCh. 14 - Prob. 13TYRCh. 14 - Motor innervation of the leg proper comes...Ch. 14 - Prob. 15TYRCh. 14 - The _______ nerves arise from the servical plexus...Ch. 14 - The crossing of a nerve fiber actract from the...Ch. 14 - Prob. 18TYRCh. 14 - Prob. 19TYRCh. 14 - Prob. 20TYRCh. 14 - Prob. 1BYMVCh. 14 - Prob. 2BYMVCh. 14 - Prob. 3BYMVCh. 14 - Prob. 4BYMVCh. 14 - State a meaning of each word element and give a...Ch. 14 - Prob. 6BYMVCh. 14 - Prob. 7BYMVCh. 14 - Prob. 8BYMVCh. 14 - Prob. 9BYMVCh. 14 - Prob. 10BYMVCh. 14 - Prob. 1WWWTSCh. 14 - Prob. 2WWWTSCh. 14 - Prob. 3WWWTSCh. 14 - Briefly explain why each of the following...Ch. 14 - Prob. 5WWWTSCh. 14 - Prob. 6WWWTSCh. 14 - Prob. 7WWWTSCh. 14 - Briefly explain why each of the following...Ch. 14 - Prob. 9WWWTSCh. 14 - Briefly explain why each of the following...Ch. 14 - Prob. 1TYCCh. 14 - Prob. 2TYCCh. 14 - Anthony gets into a fight between rival gangs. As...Ch. 14 - When a patient needs a tendon graft, surgeons...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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