EBK MICROBIOLOGY-LAB.MAN.
12th Edition
ISBN: 9780135174647
Author: CAPPUCCINO
Publisher: PEARSON
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Chapter 14, Problem 64E.4RQ
Summary Introduction
To determine: The clinical justification for the use of a rapid test procedure such as the Enterotube II System for the identification of enteric microorganisms.
Introduction: Enterobacteriaceae or enteric bacteria is a group of gram-negative bacteria that resides in the intestine of animals. These are rod-shaped, flagellated (motile), and some are non-motile. Some of them are harmless, while other species are pathogens. They are clinically important since most of the species cause diseases to animals and humans.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 14 Solutions
EBK MICROBIOLOGY-LAB.MAN.
Ch. 14 - Prob. 1QCCh. 14 - Prob. 2QCCh. 14 - Prob. 59E.1RQCh. 14 - Prob. 59E.2RQCh. 14 - Prob. 59E.3RQCh. 14 - Prob. 59E.5RQCh. 14 - Prob. 59E.6RQCh. 14 - Prob. 60E.1RQCh. 14 - Prob. 60E.2RQCh. 14 - A 6-year-old female is taken to her pediatrician...
Ch. 14 - Prob. 60E.4RQCh. 14 - How do the purposes of the bacitracin and CAMP...Ch. 14 - Prob. 62E.2RQCh. 14 - Prob. 62E.3RQCh. 14 - Prob. 62E.4RQCh. 14 - Prob. 62E.5RQCh. 14 - Prob. 63E.1RQCh. 14 - Prob. 63E.2RQCh. 14 - Prob. 63E.3RQCh. 14 - Prob. 63E.4RQCh. 14 - Prob. 64E.1RQCh. 14 - Prob. 64E.2RQCh. 14 - Prob. 64E.3RQCh. 14 - Prob. 64E.4RQCh. 14 - Prob. 65E.1RQCh. 14 - Prob. 65E.2RQCh. 14 - How would restriction enzymes play a role in...Ch. 14 - Prob. 65E.4RQCh. 14 - Prob. 66E.1RQCh. 14 - Prob. 66E.2RQCh. 14 - Prob. 66E.3RQCh. 14 - Prob. 66E.4RQCh. 14 - A male patient is diagnosed as having a urinary...Ch. 14 - Prob. 67E.1RQCh. 14 - Prob. 67E.2RQCh. 14 - Prob. 67E.3RQCh. 14 - Prob. 67E.4RQ
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