BROOKER BIOLOGY
5th Edition
ISBN: 9781307656152
Author: BROOKER
Publisher: MCG/CREATE
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Textbook Question
Chapter 14, Problem 5TY
For the lac operon, what would be the expected effects of a mutation in the operator site that prevented the binding of the repressor protein?
- a. The operon would always be turned on.
- b. The operon would always be turned off.
- c. The operon would always be turned on, except when glucose is present.
- d. The operon would be turned on only in the presence of lactose.
- e. The operon would be turned on only in the presence of lactose and the absence of glucose.
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If lactose isn't present in the environment of an E. coli, what is the state of the lac operon?
O a. It is "on" (high transcription) because will glucose will still be present, so the operon's products will be needed.
O b. It is "on" (high transcription) because CAP will be bound to the promoter
O c. It is "off" (no transcription) because RNA polymerase will be inactivated
d. It is "off" (no transcription) and the repressor will be bound to the operator
A mutation in the operator region of the trp operon can prevent the trp repressor from binding to this operator. When these mutant cells are placed in a solution containing chicken broth, which of the following happens?
a. Tryptophan would bind to the repressor.
b. The Repressor would bind to the operator irrespective of the presence of amino acids in the environment.
c. The transcription of the trip operon would be inhibited.
d. The transcription of the repressor protein is inhibited
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation
a.Which operons would be functional under these conditions?
b.What repressors would be made? (NOTE: name the repressors using their gene names)
c.Which repressor(s) would be made in the inactive form?
d.Which repressor(s) would be made in the active form?
e.Which repressor(s) under these conditions can bind the operator sequence?
f.Which repressor(s)under these conditions cannot bind the operator sequence?
Chapter 14 Solutions
BROOKER BIOLOGY
Ch. 14.1 - Prob. 1CCCh. 14.2 - Which genes are under the control of the lac...Ch. 14.2 - With regard to regulatory proteins and small...Ch. 14.2 - What were the key observations made by Jacob,...Ch. 14.2 - CoreSKILL What was the eventual hypothesis...Ch. 14.2 - Prob. 3EQCh. 14.2 - Core Skill: Connections Look back at Fig 9.12....Ch. 14.2 - What are the advantages of having both an...Ch. 14.2 - Prob. 2CSCh. 14.3 - Prob. 1CC
Ch. 14.4 - What are the two opposing effects that histone...Ch. 14.4 - Prob. 1CSCh. 14.5 - Prob. 1CCCh. 14.5 - Prob. 2CCCh. 14 - Prob. 1TYCh. 14 - Prob. 2TYCh. 14 - Transcription factors that bind to DNA and...Ch. 14 - Prob. 4TYCh. 14 - For the lac operon, what would be the expected...Ch. 14 - Prob. 6TYCh. 14 - The trp operon is considered _____ blank operon...Ch. 14 - Prob. 8TYCh. 14 - Prob. 9TYCh. 14 - _____ blank refers to the process that allows a...Ch. 14 - Prob. 1CQCh. 14 - Transcriptional regulation often involves a...Ch. 14 - Prob. 3CQCh. 14 - Discuss the advantages and disadvantages of...Ch. 14 - Discuss the advantages and disadvantages of...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.arrow_forwardStrain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind allolactose. Otherwise all other parts of the operon are functional. 1. Can strain LOLZ metabolize lactose if it is present? 2. If lactose is absent, will strain LOLZ transcribe its lac operon? 3. If lactose is present, will strain LOLZ transcribe its lac operon?arrow_forwardHow does the lac operon regulate lactose digestion in bacteria?a. The repressor protein becomes a lactose-digesting enzyme onlywhen lactose is present.b. The repressor protein binds to the lac operon when lactose ispresent, blocking transcription.c. When lactose is present, it binds to the operator region of the lacoperon, activating transcription of the repressor protein gene.d. The repressor protein falls off the lac operon when lactose ispresent, and lactose-digesting genes are expressed.arrow_forward
- Which statement about the transcription attenuation mechanism is TRUE? 1.In some operons (e.g., the his operon), attenuation may be the only regulatory mechanism. 2. Sequences of the trp operon leader RNA resemble an operator. 3. The leader peptide acts by a mechanism that is similar to that of a repressor protein. 4.The leader peptide gene of the trp operon includes no Trp codons. 5. The leader peptide is an enzyme that catalyzes transcription attenuation.arrow_forwardAnswer this 3 part question: (MC) What is the function of lactose in regulation of the lac operon? 1. activates a repressor protein 2. activates an activator protein 3. inactivates a repressor protein 4. inactivates an activator protein Consider a bacterial strain with a lac operon Р. О Y A a) Mention which parts of the DNA region shown in the diagram encode for • Enzyme proteins? • Operator • Promoter • Repressor gene b) Where would the lac repressor be bound in a E. coli cell that is growing in ( • high lactose? No lactosearrow_forwardA mutation at the operator prevents the regulator protein from binding. What effect will this mutation have in the following types of operons? a. Regulator protein is a repressor of a repressible operon. b. Regulator protein is a repressor of an inducible operon.arrow_forward
- What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardIf a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or glucose, how many proteins (and which ones) are bound to the lac operon? b. Without lactose, but with glucose, how many proteins (and which ones) are bound to the lac operon??- lac repressorarrow_forwardIn the lac operon, how would gene expression be affected if each one of the following segments was missing? A. lac operon promoter B. Operator site C. lacA genearrow_forward
- For the trp operon, determine whether genes will be expressed (on) or silenced (off) given the following: a. Tryptophan levels are high. b. The trp repressor can no longer bind tryptophan.arrow_forwardDecide which operon each of the following characteristics applies to. Note: a description may apply to both. a. lac operon b. trp operon 68. repressible 69. Turned off when end product is present (feedback inhibition) 70. Promoter, activator region, and 3 genes 71. Repressible 72. contain operator and promoter regions as well as transcript units of at least two or more coding genes 73. Codes for catabolic enzymes that break down lactose 74. Inducible 75. Promoter, activator region, and 5 genes 76. Codes for anabolic enzymes that help manufacture tryptophan 77. Lactose is the co-inducer 78. Turned on when substrate is present 79. Tryptophan is the co-repressor 80. Regulatory gene segmentsarrow_forwardMutations may have an effect on the expression of the lac operon and the trp operon. Would the following mutations have a cis- or transeffect on the expression of the protein-encoding genes in the operon? A. A mutation in the operator site that prevents lac repressor from binding to it B. A mutation in the lacI gene that prevents lac repressor from binding to DNA C. A mutation in the trpL gene that prevents attenuationarrow_forward
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