Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 14, Problem 5CT
If a bacterium cannot use citrate, what result will occur in citrate agar?
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Why does motility media contain a lower amount of agar than ordinary agar?
A bacterial organism is used to inoculate mannitol broth medium. Following incubation the broth is yellow. This indicates that the organism:
A) can use lactose and glucose
B) cannot use lactose
C) can use mannitol
D) cannot use mannitol
Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow
Chapter 14 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 14 - Why are fermentation tubes evaluated at 24 and 48...Ch. 14 - If an organism metabolizes glucose aerobically,...Ch. 14 - Which of these media is selective?...Ch. 14 - Could an organism be a fermenter and also be both...Ch. 14 - How could you determine which of the following...Ch. 14 - If a bacterium cannot ferment glucose, why not...Ch. 14 - If a bacterium cannot use glucose, what result...Ch. 14 - If a bacterium cannot use citrate, what result...Ch. 14 - You put an opened bottle of orange drink in the...
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- Provide the conditions (e.g., intrinsic properties, storage conditions, etc.) that will make the recovery of the following microorganisms in commercially sterile foods acceptable: a) Obligate thermophilic sporeforming bacteria b) Acid-tolerant microorganisms c) Mesophilic or thermophilic sporeformersarrow_forwardIn a nutrient broth, where do microorganisms acquire their requirements for mineral elements like Ca, Fe, etc. from? Can gelatin be used in culture media preparation? How is it different from agar? During sterilization, pH of culture media may change. How can this be fixed? How does pH affect the shelf life of unsterilized media?arrow_forwardAgar is a solidifying agent used in media preparation. What is its origin?arrow_forward
- Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most microorganisms. Why does this property make agar an excellent solidifying agent for microbiological media?arrow_forwardMacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.arrow_forwardwhy is it important that a small amount of inoculum be used in preparation of an agar slant?arrow_forward
- Why do we grow the bacteria in minimal media when testing for citrate utilization?arrow_forwardExplain the function of the ferrous ammonium sulfate in SIM agar.arrow_forwardAn unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated that same unknown onto the Mannitol salt agar media?arrow_forward
- MacConkey Agar Lactose fermenter or non-fermenter: ___________________________ Gram reaction based on MAC Growth: ___________________________ Coliform or non-coliform: _____________________________________arrow_forwardIf the result of an unknown bacteria is “poor growth” and “red growth,” can this result be interpreted as “organism does not ferment mannitol?” Explain why or why not. If the result of an unknown bacteria is “good growth” and “yellow growth,” is the unknown bacteria more likely Staphylococcus aureus or Staphylococcus epidermidis?arrow_forwardListen The following is a recipe for a bacterial growth medium: 35 g Dextrose, 12 g Agar, 1.2g Salt, 3 g Yeast cell extract, 20g tryptone. Based on the recipe, what type of growth medium would this be? A) Chemically defined growth medium B) Minimal growth medium C) Complex growth medium D) Chemically undefined growth medium E) More than one of the answers is correct.arrow_forward
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