Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 14, Problem 3CT
If a bacterium cannot ferment glucose, why not test its ability to ferment other carbohydrates?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
If the result of an unknown bacteria is “poor growth” and “red growth,” can this result be interpreted as “organism does not ferment mannitol?” Explain why or why not.
If the result of an unknown bacteria is “good growth” and “yellow growth,” is the unknown bacteria more likely Staphylococcus aureus or Staphylococcus epidermidis?
A bacterium requires only the amino acid methionineas an organic nutrient and lives in lightless caves. Whatmode of nutrition does it employ? Explain.
You are given a mixed culture of 3 bacteria with following characteristics-
1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4
sugars but forms endospores and is sensitive to penicillin)
2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce
carbon dioxide gas on fermenting these
3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose
and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.)
Also they all give similar looking white-colored colonies on an agar plate.
Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools
that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as
confirm them. Any other technique that you use should be one you learned in lab. No need to give
detailed info about how to perform the technique but it…
Chapter 14 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 14 - Why are fermentation tubes evaluated at 24 and 48...Ch. 14 - If an organism metabolizes glucose aerobically,...Ch. 14 - Which of these media is selective?...Ch. 14 - Could an organism be a fermenter and also be both...Ch. 14 - How could you determine which of the following...Ch. 14 - If a bacterium cannot ferment glucose, why not...Ch. 14 - If a bacterium cannot use glucose, what result...Ch. 14 - If a bacterium cannot use citrate, what result...Ch. 14 - You put an opened bottle of orange drink in the...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Create an experimental flow chart for the extraction of glucose-1-phosphate from starch.arrow_forwardDo these organisms test for negative or positive in a starch hydrolysis test?arrow_forwardYeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L. The growth mediğim in the reactor, which is well-mixed so the cells have access to all nutrients, contains 150 g CELL DATA ribose (C5H10O5, MW 150), 75 g ammonia (NH3, MW 17), and 85 g oxygen (O2, MW 32). A-10. Determine the maximum concentration of cells, [X], that will form in the bioreactor when the limiting nutrient is consumed. Search Yx/ribose YX/02 YX/NH3 Specific growth rate on limiting nutrient, u Lag phase duration Deceleration phase duration 0.48 g/g 0.87 g/g 0.23 g/g 0.51 h 30 minutes Negligiblearrow_forward
- Why are some microorganisms capable of utilizing certain carbohydrates and some are not?arrow_forwardTwo cultures of a facultative anaerobe are grown in the same medium, but one culture is exposed to air and the other is maintained under anaerobic conditions. Which of the two cultures will contain more cells at the end of a week? Why?arrow_forwardexplain why there is little to no growth of these organisms in the minimal salt media with glucose compared to the complex media. (HINT: these organisms are chemoheterotrophs and need an organic source of broth carbon and energy)...arrow_forward
- Bread making involves ethanol fermentation and typically uses yeast, sugar,flour, and water. Why are yeast and sugar used?arrow_forwardDescribe the kind of food that would have lactic acid bacteria. Mention their useful applications.arrow_forwardE. coli, a facultative anaerobe, is grown for 24 hours on the same type of solid medium, but under two different conditions: one aerobic, the other anaerobic. The size of the colonies would bea) the same under both conditions.b) larger when grown under aerobic conditions.c) larger when grown under anaerobic conditions.arrow_forward
- Why do some bacteria grow in the presence of salts?arrow_forwardWhat type of metabolism is occurring at the top of the Fluid Thioglycolate Medium tube? a. The organism will use either aerobic or anaerobic metabolism. b. The organism will be using anaerobic metabolism. c. The organism will be using aerobic metabolism.arrow_forwardRead the following scenario and answer the associated questions. Hydrogen peroxide was added to a piece of potato and potatoes contain the enzyme, catalase. When hydrogen peroxide was added to the potato, bubbles appeared due to the presence of oxygen gas. The presence of the oxygen gas indicates that the catalase was breaking down the hydrogen peroxide into oxygen gas and water. If the hydrogen peroxide was removed and re-added to the potato, would bubbling occur again? Explain why or why not What do you think would happen if the potato in the above question was boiled before being placed into a container with hydrogen peroxide? Why is the reusability of enzymes important?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Drug Abuse, Causes, Signs and Symptoms, Diagnosis and Treatment.; Author: Medical Centric;https://www.youtube.com/watch?v=b6Dte96WdqM;License: Standard youtube license