Study Guide with Student Solutions Manual and Problems Book for Garrett/Grisham's Biochemistry, 6th
Study Guide with Student Solutions Manual and Problems Book for Garrett/Grisham's Biochemistry, 6th
6th Edition
ISBN: 9798214167343
Author: GARRETT
Publisher: Cengage Learning US
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Chapter 14, Problem 2P

Answers to all problems are at the end οΓthis book. Detailed solutions are available in the Student Solutions Manual. Study Guide, and Problems Book.

Using Site-Direcled Muta.nts to Understand an Enzyme Mechanism In this chapter, the exponent in which Craik and Rutter replaced Asp102 with Asn in trypsin (reducing activity 10,000 -fold) was discussed.

  1. On the basis of your knowledge of the catalytic triad structure in trypsin, suggest a structure for the “uncatalytic triad" of Asn-His-Ser in this mutant enzyme.
  2. Explain why the structure you have proposed explains the reduced activity of the mutant trypsin.
  3. See the original journal articles (Sprang, et al., 1987. Science 237:905-913) to Craik, et al., 1987. Scieence 237:909-913) to see Craik and Rutter's answer to this question.

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12. When glutamic acid is heated to 180°C., it loses a molecule of water to form a lactam. The bond that creates the lactam is an amide bond that forms between the amino nitrogen and the carbon of the side chain -COOH group. What is the most reasonable structure for this lactam? (NOTE: The structure of glutamic acid is shown in Question 1.) COOH COOH ا کچھ جو کچھ C D COOH B COOH E COOH
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