GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS
6th Edition
ISBN: 9781260239775
Author: BROOKER
Publisher: MCG
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Chapter 14, Problem 2EQ
Chapter 21 describes a blotting method known as Northern blotting, which can be used to detect RNA transcribed from a particular gene or a particular operon. In this method, a specific RNA is detected by using a short segment of cloned DNA as a probe. The DNA probe is complementary to the RNA that the researcher wishes to detect. After the probe DNA binds to the RNA within a blot of a gel, the RNA is visualized as a dark band. For example, a DNA probe complementary to the mRNA of the lac operon could be used to specifically detect the lac operon mRNA on a gel blot. As shown here, the method of Northern blotting can be used to determine the amount of a particular RNA transcribed under different types of growth conditions. In this Northern blot, bacteria containing a normal lac operon were grown under different types of conditions, and then the mRNA was isolated from the cells and subjected to Northern blotting, using a probe that is complementary to the mRNA of the lac operon.
Based on your understanding of the regulation of the lac operon, explain these results. Which is more effective at shutting down the lac operon, the binding of lac repressor or the removal of CAP? Explain your answer based on the results shown in the Northern blot.
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Chapter 14 Solutions
GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS
Ch. 14.1 - 1. A repressor is a __________ that _________...Ch. 14.1 - 2. Which of the following combinations will cause...Ch. 14.2 - 1. What is an operon?
a. A site in the DNA where...Ch. 14.2 - The binding of _______ to lac repressor causes lac...Ch. 14.2 - Prob. 3COMQCh. 14.2 - How does exposing an E. coli cell to glucose...Ch. 14.3 - When tryptophan binds to trp repressor, this...Ch. 14.3 - During attenuation, when tryptophan levels are...Ch. 14.3 - Operons involved with the biosynthesis of...Ch. 14.4 - 1. Translation can be regulated by
a....
Ch. 14.4 - Prob. 2COMQCh. 14.5 - 1. For a riboswitch that controls transcription,...Ch. 14 - 1. What is the difference between a constitutive...Ch. 14 - In general, why is it important to regulate genes?...Ch. 14 - Prob. 3CONQCh. 14 - Transcriptional regulation often involves a...Ch. 14 - An operon is repressible-a small effector molecule...Ch. 14 - Prob. 6CONQCh. 14 - 7. What is enzyme adaptation? From a genetic point...Ch. 14 - Prob. 8CONQCh. 14 - Prob. 9CONQCh. 14 - Prob. 10CONQCh. 14 - Mutations may have an effect on the expression of...Ch. 14 - 12. Would a mutation that inactivated lac...Ch. 14 - Prob. 13CONQCh. 14 - 14. As shown in Figure 14.12, four regions within...Ch. 14 - 15. As described in Chapter 13, enzymes known as...Ch. 14 - 16. The combination of a 3–4 stem-loop and a...Ch. 14 - 17. Mutations in tRNA genes can create tRNAs that...Ch. 14 - Prob. 18CONQCh. 14 - Prob. 19CONQCh. 14 - A species of bacteria can synthesize the amino...Ch. 14 - 21. Using three examples, describe how allosteric...Ch. 14 - How are the actions of lac repressor and trp...Ch. 14 - 23. Transcriptional repressor proteins (e.g., lac...Ch. 14 - Answer the following questions that pertain to the...Ch. 14 - Chapter 21 describes a blotting method known as...Ch. 14 - As described in experimental question E2 and also...Ch. 14 - Prob. 4EQCh. 14 - Explain how the data shown in Figure 14.9 indicate...Ch. 14 - Prob. 6EQCh. 14 - Lets suppose you have isolated a mutant strain of...Ch. 14 - Prob. 1QSDCCh. 14 - Prob. 2QSDC
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