Essential Cell Biology 5e
Essential Cell Biology 5e
5th Edition
ISBN: 9780393691108
Author: Bruce Alberts, Karen Hopkin, Alexander D Johnson, David Morgan, Martin Raff, Keith Roberts, Peter Wa
Publisher: W. W. Norton
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Chapter 14, Problem 18Q

A.

Summary Introduction

To explain: What would be the effect if a purified bacteriorhodopsin and purified ATP synthase from ox-heart mitochondria together is reconstituted to the same membrane vesicles and then the ADP, Pi, and light are added to the external medium.

Concept introduction:

Bacteriorhodopsin present in archeal cells is a protein that acts as a proton pump that can pump protons across the membrane in the presence of light energy. ATP synthase is an ion pump, molecular motor, and is an enzyme that catalyzes the production of ATP. These two different proteins from the different sources are added to the vesicle, and ATP is produced in the vesicle.

B.

Summary Introduction

To explain: What would be the effect if not all the detergent are removed and the vesicle membrane therefore remains leaky to ions.

Concept introduction:

Bacteriorhodopsin present in archeal cells is a protein that acts as a proton pump that can pump protons across the membrane in the presence of light energy. ATP synthase is an ion pump, molecular motor, and is an enzyme that catalyzes the production of ATP. These two different proteins from the different sources are added to the vesicle, and ATP is produced in the vesicle.

C.

Summary Introduction

To explain: The reasons to defend the friends statement “Why would anybody want to mix vanilla pudding with brake fluid’’ about the new experiment.

Concept introduction:

Bacteriorhodopsin present in archeal cells is a protein that acts as a proton pump that can pump protons across the membrane in the presence of light energy. ATP synthase is an ion pump, molecular motor, and is an enzyme that catalyzes the production of ATP. These two different proteins from the different sources are added to the vesicle, and ATP is produced in the vesicle.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…
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