EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 14, Problem 12MC
Summary Introduction
Introduction:
Epidemiology is the branch of medical science that deals with the study of disease and its causative agents. Epidemiologists deal with the disease outbreak and preventive control measures to tackle it down. Index of infected people and victims by a disease outbreak is surveyed by the epidemiologist to have a track on the record report.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 14 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 14 - Prob. 1TMWCh. 14 - A Deadly Carrier In 1937, a man employed to lay...Ch. 14 - Prob. 2TMWCh. 14 - Prob. 3TMWCh. 14 - Why is mutated Streptococcus pneumoniae, which...Ch. 14 - Prob. 5TMWCh. 14 - Prob. 6TMWCh. 14 - TB in the Nursery In the early fall, a neonatal...Ch. 14 - Why is an acute disease with a high rate of...Ch. 14 - Prob. 1EDCS
Ch. 14 - Prob. 8TMWCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - The most frequent portal of entry for pathogens is...Ch. 14 - Prob. 5MCCh. 14 - Prob. 6MCCh. 14 - Prob. 7MCCh. 14 - Prob. 8MCCh. 14 - Prob. 9MCCh. 14 - Prob. 10MCCh. 14 - Which of the following statements is the best...Ch. 14 - Prob. 12MCCh. 14 - Prob. 13MCCh. 14 - Prob. 14MCCh. 14 - Prob. 15MCCh. 14 - Prob. 1FIBCh. 14 - Fill in the Blanks 2. Infections that may go...Ch. 14 - Prob. 3FIBCh. 14 - Prob. 4FIBCh. 14 - Prob. 5FIBCh. 14 - Prob. 6FIBCh. 14 - Prob. 7FIBCh. 14 - Prob. 8FIBCh. 14 - Prob. 9FIBCh. 14 - Prob. 10FIBCh. 14 - Prob. 1SACh. 14 - Prob. 2SACh. 14 - Prob. 3SACh. 14 - List Kochs four postulates, and describe...Ch. 14 - Prob. 5SACh. 14 - Prob. 6SACh. 14 - Describe the parenteral route of infection.Ch. 14 - In general, contrast transient microbiota with...Ch. 14 - Contrast the terms infection and morbidity.Ch. 14 - Prob. 10SACh. 14 - Each map below shows the locations (dots) of cases...Ch. 14 - Prob. 2VICh. 14 - Explain why Ellen H., a menopausal woman, may have...Ch. 14 - Prob. 2CTCh. 14 - A 27-year-old woman went to her doctors office...Ch. 14 - Over 30 children younger than three years of age...Ch. 14 - Prob. 5CTCh. 14 - Corals are colonial marine animals that feed by...Ch. 14 - If a mutation occurred in Escherichia coli that...Ch. 14 - Using the following terms, fill in the following...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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