EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 14, Problem 7SA
Describe the parenteral route of infection.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 14 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 14 - Prob. 1TMWCh. 14 - A Deadly Carrier In 1937, a man employed to lay...Ch. 14 - Prob. 2TMWCh. 14 - Prob. 3TMWCh. 14 - Why is mutated Streptococcus pneumoniae, which...Ch. 14 - Prob. 5TMWCh. 14 - Prob. 6TMWCh. 14 - TB in the Nursery In the early fall, a neonatal...Ch. 14 - Why is an acute disease with a high rate of...Ch. 14 - Prob. 1EDCS
Ch. 14 - Prob. 8TMWCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - The most frequent portal of entry for pathogens is...Ch. 14 - Prob. 5MCCh. 14 - Prob. 6MCCh. 14 - Prob. 7MCCh. 14 - Prob. 8MCCh. 14 - Prob. 9MCCh. 14 - Prob. 10MCCh. 14 - Which of the following statements is the best...Ch. 14 - Prob. 12MCCh. 14 - Prob. 13MCCh. 14 - Prob. 14MCCh. 14 - Prob. 15MCCh. 14 - Prob. 1FIBCh. 14 - Fill in the Blanks 2. Infections that may go...Ch. 14 - Prob. 3FIBCh. 14 - Prob. 4FIBCh. 14 - Prob. 5FIBCh. 14 - Prob. 6FIBCh. 14 - Prob. 7FIBCh. 14 - Prob. 8FIBCh. 14 - Prob. 9FIBCh. 14 - Prob. 10FIBCh. 14 - Prob. 1SACh. 14 - Prob. 2SACh. 14 - Prob. 3SACh. 14 - List Kochs four postulates, and describe...Ch. 14 - Prob. 5SACh. 14 - Prob. 6SACh. 14 - Describe the parenteral route of infection.Ch. 14 - In general, contrast transient microbiota with...Ch. 14 - Contrast the terms infection and morbidity.Ch. 14 - Prob. 10SACh. 14 - Each map below shows the locations (dots) of cases...Ch. 14 - Prob. 2VICh. 14 - Explain why Ellen H., a menopausal woman, may have...Ch. 14 - Prob. 2CTCh. 14 - A 27-year-old woman went to her doctors office...Ch. 14 - Over 30 children younger than three years of age...Ch. 14 - Prob. 5CTCh. 14 - Corals are colonial marine animals that feed by...Ch. 14 - If a mutation occurred in Escherichia coli that...Ch. 14 - Using the following terms, fill in the following...
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