Chapter 13.4, Problem 1CC

Summary Introduction

To identify:

The sequence and the types of the bonds that are being cleaved in the sequence of the DNA by the restriction enzyme PvuI namely:

$\begin{array}{l}\text{5'-CGATCG-3'}\\ \text{3'-GCTAGC-5'}\end{array}$

Concept introduction:

The enzyme PvuI cuts in between the T-C nucleotide in the sequence given. The sequence after the digestion of the restriction enzyme sequence will be:

$\begin{array}{l}\text{5'-CGAT--CG-3'}\\ \text{3'-GC--TAGC-5'}\end{array}$

The types of the bonds that will be cleaved in between the nucleotides are the phosphate bonds between the pentose sugar and the nucleotide connecting the next nucleotide in the sequence.