Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Textbook Question
Chapter 13, Problem 3TYU
In analyzing the number of different bases in a DNA sample, which result would be consistent with the base-pairing rules?
- A. A=G
- B. A + G = C + T
- C. A+ T = G + C
- D. A=C
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What is the purpose of Bromophenol Blue in electrophoresis?
a.
To follow the progress of the gel as it is running
b.
It acts as a DNA size ladder or DNA size standards.
c.
To allow the electric current to move through the gel.
d.
To visualize the DNA after the gel has finished running.
What is the purpose of acetate in TAE?
a.
It helps to maintain the pH of the buffer
b.
To carry electrons to allow the electric current to travel through the gel.
c.
It is used to stop the action of DNases
d.
It is required to see the DNA on a UV light.
When working with the gel electrophoresis chamber, what must we keep in mind?
A. Keeping the gel flat on the table without any buffer solution
B. Not changing your pipette in between DNA samples
C. Not shaking the chamber after the DNA has been loaded
D. Loading two DNA samples per a single well
Choose the combination of answers that most accurately completes the statement. Why must the lagging strand of DNA be replicated in short pieces? a. because of limited space b. otherwise, the helix will become distorted c. the DNA polymerase can synthesize in only one direction d. to make proofreading of code easier
Chapter 13 Solutions
Campbell Biology in Focus
Ch. 13.1 - Given a polynucleotide sequence such as GAATTC,...Ch. 13.1 - Prob. 2CCCh. 13.2 - What role does base pairing play in the...Ch. 13.2 - Make a table listing the functions of seven...Ch. 13.2 - MAKE CONNECTIONS What is the relationship between...Ch. 13.3 - Describe the structure of a nucleosome, the basic...Ch. 13.3 - What two properties, one structural and one...Ch. 13.4 - Prob. 1CCCh. 13.4 - DRAW IT One strand of a DNA molecule has the...Ch. 13.4 - Describe the role of complementary base pairing...
Ch. 13 - In his work with pneumonia-causing bacteria and...Ch. 13 - Prob. 2TYUCh. 13 - In analyzing the number of different bases in a...Ch. 13 - The elongation of the leading strand during DNA...Ch. 13 - Prob. 5TYUCh. 13 - Prob. 6TYUCh. 13 - Prob. 7TYUCh. 13 - Prob. 8TYUCh. 13 - Prob. 9TYUCh. 13 - MAKE CONNECTIONS Although the proteins that cause...Ch. 13 - Prob. 11TYUCh. 13 - FOCUS ON EVOLUTION Some bacteria may be able to...Ch. 13 - FOCUS ON ORGANIZATION The continuity of life is...Ch. 13 - Prob. 14TYU
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- Choose the combination of answers that most accurately completes the statement.Why must the lagging strand of DNA be replicated in short pieces? a. because of limited space b. otherwise, the helix will become distorted c. the DNA polymerase can synthesize in only one direction d. to make proofreading of code easierarrow_forwardWhy do the fragments of DNA in gel electrophoresis travel away from the negative electrode? A. DNA is positively charged to attracted to the negative end of the unit B. the agarose gel is positively charged C. the agarose gel in negatively charged D. DNA is negatively charged so attracted to the positive end of the unitarrow_forwardWhen two adjacent bases in the same strand of DNA dimerize (form a covalent bond between them), what happens to the DNA? a. the original strand of DNA now contains a new DNA sequence b. the original strand of DNA is prevented from opening during replication, so this section of DNA will not be replicated c. the original strand of DNA is methylated, which causes the bases to mismatch d. the original strand of DNA is kinked, which prevents DNA polymerase from working properly e. the original strand of DNA is unaffected, so no additional mutations arisearrow_forward
- You are studying bacterial cells and you find bacteria with a mutation that causes it to produce non-functional ligase. If you allowed that cell to replicate once and then you isolated the DNA from the two newly formed cells what would you expect to find? A. One longer single strand of DNA and many short single strands of DNA B. Two longer single strands of DNA and many short single strands of DNA C. Four longer single strands of DNA and many short single strands of DNA D. Eight longer single strands of DNA and many short single strands of DNA Choose the correct answer above and explain why you chose that answer. Your explanation should discuss the replication of DNA in bacteria and the role of ligase in DNA replication.arrow_forwardWhich of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling b.metacoiling c. ultracoiling d. telephone coilingarrow_forwardWhy are mutations more likely to occur in repeated DNA sequences? a. These bases are unstable b. bases in the strand can form base pairs, generating loops that interfere with replication and repair enzymes. c. The repeats hold onto the replication enzymes, causing base mismatches d. the repeats attract and bind to mutagens, increasing the mutation ratearrow_forward
- There is segment of double-stranded DNA that is found to have a total of 140 bases. If 45 of those bases are Thymine, how many of them are Cytosine? Group of answer choices a. 50 b. 25 c. 45 d. 90arrow_forwardUse the gel diagram below to answer Questions 62 and 63. The positively-charged electrode of the electrophoresis gel is shown at the bottom of the diagram; the negatively-charged electrode is at the top. The mixture in each well included the original DNA fragment, all four dNTPs, one type of ddNTP as labeled in the diagram, and other necessary components of the dideoxy chain-termination reaction. The results are below. ddATP ddGTP ddCTP ddTTP (A) (B) (C) (D) (E) +. 62. Which of the following represents the sequence of the template strand?" A. 5'--GAAGACTAACATTCA--3' B. 5'-- ACTTACAATGTAGUA--3' C. 5'-- CTTCTGATTGTAAGT--3' D. 5'--ACTTACAATCAGAAG--3' E. 5'--TGAATGTTAGTCTTC--3'arrow_forwardWhat term is used to describe the fact that the 5' end of one strand lines up with the 3' end of the complimentary strand? a. Antiverted b. Cross listed c. Reverse d. Antiparallelarrow_forward
- A primer is laid down complementary to the DNA sequence TAGCAATCGCA to prime synthesis of a daughter strand. What will the sequence of the primer be? A. ATCGTTAGCGT B. ATGGTTAGGGT C. AUCGUUAGCGU D. AUCGTUAGCGTarrow_forwardHow is each new nucleotide added to the growing end of a DNA strand? a. A dehydration reaction joins the monomer to the new strand b. By using DNA polymerase c. Both of the above d. A hydrolytic reaction joins the monomer to the new strandarrow_forwardWhat is the role of CTAB in DNA isolation? A. Neutralize negative charge of DNA B Precipitate DNA C Remove polyphenols and tannins D Precipitate polysaccharidesarrow_forward
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