Chapter 13.2, Problem 5MI

Summary Introduction

To fill:

The correct terms in the given blanks to complete the sentence.

Introduction:

DNA, or deoxyribonucleic acid, is the genetic material for most animals and plants, and even some viruses.

Answer to Problem 5MI

The correct term is given in the table:

S. No.	Term
1.	Restriction enzymes
2.	Gel electrophoresis
3.	Restriction enzymes
4.	Sticky ends
5.	EcoRI
6.	Sticky ends
7.	Restriction enzymes
8.	Blunt ends
9.	Blunt ends

Explanation of Solution

Scientists uses restriction enzymes to cut DNA at specific sequences, and gel electrophoresis to separate fragments based on size. Some restriction enzymes create DNA with single-stranded, sticky ends. Eco RI is an example of this type of enzyme. The resulting DNA fragments can be joined with other DNA fragments that have complementary sticky ends. Other restriction enzymes create blunt ends, which can be joined to another DNA fragment that has blunt ends.