Campbell Biology In Focus
2nd Edition
ISBN: 9780134203072
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Textbook Question
Chapter 13, Problem 4TYU
The elongation of the leading strand during DNA synthesis
- A. progresses away from the replication fork.
- B. occurs in the 3’ → 5’ direction.
- C. produces Okazaki fragments.
- D. depends on the action of DNA polymerase.
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During DNA replication, the leading strand is synthesized continuously, while the lagging strand is synthesized as Okazaki fragments. This is because:
O A. DNA polymerases can bind to only one strand at a time.
O B. Two different DNA polymerases are involved in replication (DNA polymerase I and II).
O C. DNA synthesis can take place only in the 5 -> 3 direction.
D. The primers are made of RNA.
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For the statements below, indicate whether the statement applies to the leading strand, or to the lagging strand, or to both.
a. synthesized in the 5'→3' direction
b. synthesized continuously
c. require(s) DNA ligase to join fragments
d. described as a daughter strand
e. synthesized in the same direction as the movement of the replication fork
f. DNA polymerase is the enzyme involved in forming this polynucleotide.
Match the enzymes provided from (1-4) in the list of choices with their matching function (A-D) during DNA replication.
A. Disrupts hydrogen bonds between DNA bases
B. Can only add nucleotides to an existing 3 OH end
C. Can't add nucleotides to a chain, but can make covalent bonds
D. Actually a specialized form of RNA polymerase
select
1. DNA polymerase
select
2. Primase
select
3. Ligase
select v 4. Helicase
Chapter 13 Solutions
Campbell Biology In Focus
Ch. 13.1 - Given a polynucleotide sequence such as GAATTC,...Ch. 13.1 - Prob. 2CCCh. 13.2 - What role does base pairing play in the...Ch. 13.2 - Make a table listing the functions of seven...Ch. 13.2 - MAKE CONNECTIONS What is the relationship between...Ch. 13.3 - Describe the structure of a nucleosome, the basic...Ch. 13.3 - What two properties, one structural and one...Ch. 13.4 - Prob. 1CCCh. 13.4 - DRAW IT One strand of a DNA molecule has the...Ch. 13.4 - Describe the role of complementary base pairing...
Ch. 13 - In his work with pneumonia-causing bacteria and...Ch. 13 - What is the basis for the difference in how the...Ch. 13 - In analyzing the number of different bases in a...Ch. 13 - The elongation of the leading strand during DNA...Ch. 13 - Prob. 5TYUCh. 13 - Prob. 6TYUCh. 13 - Prob. 7TYUCh. 13 - Prob. 8TYUCh. 13 - Prob. 9TYUCh. 13 - MAKE CONNECTIONS Although the proteins that cause...Ch. 13 - Prob. 12TYUCh. 13 - FOCUS ON EVOLUTION Some bacteria may be able to...Ch. 13 - FOCUS ON ORGANIZATION The continuity of life is...
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- The function of DNA ligase is to: a. Catalyze formation of phosphodiester bonds between adjacent nucleotides b. Catalyze formation of hydrogen bonds between adjacent nucleotides c. Keep single strands of DNA apart during replication d. Facilitate base pairing between single stranded molecules in DNA e. Both a. and d. are correctarrow_forwardDNA polymerase III is a processive enzyme, which means that a. it does not dissociate from the growing strand after it has attached a nucleotide to the 3′ end. b. it makes a new strand very quickly. c. it proceeds toward the opening of the replication fork. d. it copies DNA with relatively few errors.arrow_forwardThe enzyme responsible for adding new nucleotides to a growing DNA chain during DNA replication is a. helicase. b. RNA polymerase. c. DNA polymerase. d. DNA ligase.arrow_forward
- During DNA replication, short RNA primers are made by the Primase. Why? a. To provide a 3'-OH so DNA polymerase can begin DNA synthesis. b. To recruit single stranded binding proteins to the correct location. c. To identify the termination sequence for DNA polymerase during DNA synthesis. d. To provide a 3'-OH so RNA polymerase can begin DNA synthesis. e. To identify the origin of replication to recruit the origin replication complex to the correct genomic location.arrow_forwardWhich statement about Okazaki fragments is true? Select one: a. DNA polymerase doesn’t need a primer to build these fragments b. They act as a primer that initiates DNA replication. c. They correct errors made during earlier phases of DNA replication. d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork. e. They prevent the ends of chromosomes from shortening with every replication.arrow_forwardOne of the mechanisms that leads to the DNA mutations is a process known as DNA polymerase slippage which occurs during DNA replication. Which of the following statements about DNA polymerase slippage is correct? A. Backward slippage in the newly synthesized strand will lead to an insertion mutation. B. DNA polymerase slippage can lead to a point or substitution mutation. C. Forward slippage in the template strand will lead to an insertion mutation. D. All of the abovearrow_forward
- a. unwinds the DNA helix b. stabilizes and stops the two strands from annealing (rebinding with each other) c. recoils the DNA d. cleaves both strands of DNA to relieve tension at supercoils e. places RNA primers at their proper location on the template strands f. acts as starting points for DNA polymerase g. adds DNA nucleotides to form new DNA strands h. forms phosphodiester bonds to join Okazaki fragments Esc 1. single-strand binding protein 2. helicase 3. DNA ligase 4. RNA primer 5. gyrase 6. DNA polymerase 53°F Cloudy 3 Q Search $ F5 FRarrow_forwardDNA replication is said to be semiconservative because a. one of the new molecules conserves both of the original DNA strands. b. the new DNA molecule contains two new DNA strands. c. both of the new molecules contain one new strand and one old strand. d. DNA polymerase conserves both of the old strands.arrow_forwardDuring DNA replication, which of the following steps occurs first? a. synthesis of the leading strand b. synthesis of the lagging strand c. unwinding of the parental DNA duplex d. sealing of the nicks between short DNA e. synthesis of primersarrow_forward
- Determine whether each statement isTRUE or FALSE.a. In eukaryotic chromosomes, DNA replication begins at a single point in the chromosome and proceeds in two directions.b. The bonds between the sugars and phosphates are broken during DNA replication.c. The elongation of the leading strand during DNA synthesis progresses away from the replication fork.d. Each DNA molecule resulting from replication has one original strand and one new strand.e. The difference in how the leading and lagging strands of DNA molecules are synthesized is due to DNA polymerase adding new nucleotides only to the 3’ end of a growing strand, and the strands are anti-parallel.arrow_forwardWhich of the following is NOT true of DNA replication? a. Helicase uses ATP as an energy source. b. Primase uses ATP to build primers. c. Single-stranded binding proteins are enzymes that hold the strands open. d. Telomerase adds nucleotides to the original DNA template. e. Ligase uses a condensation reaction to make a single phosphodiester bond at each site of ligation..arrow_forwardA drug that inhibits the DNAa protein is added to a culture of E. coli cells. What is the first step of DNA replication that will be impaired by this drug? PICK ONE ANSWER: A. Keeping parent DNA strands separated B. Releasing tension generated by supercoiling C. Activating the origin of replication D. Making primers E. Forming phosphodiester bondsarrow_forward
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