Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 13, Problem 4P
Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
Graphing the Results from Kinetics Experiments with Enzyme Inhibitors The following kinetic data were obtained for an enzyme in the absence of any inhibitor (1), and in the presence of two different inhibitors (2) and (3) at 5 mM concentration. Assume [ET] is the same in each experiment.
Graph these data as Lineweaver-Burk plots and use your graph to find answers to a. and b.
a. Determine Vmax and Km for the enzyme.
b. Determine the type of inhibition and the K1 for each inhibitor.
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The rate data from an enzyme catalyzed reaction with and without an inhibitor present is found in the image.
Question: what is the KM and Vm and the nature of inhibition
1. Estimate the concentration of an enzyme within a living cell. Assume that:
(a): fresh tissue is 80% water and all of it is intracellular
(b): the total soluble protein represents 15% of the weight
(c): all the soluble proteins are enzymes
(d): the average molecular weight of the proteins is 150,000
(E): about 100 different enzymes are present
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Chapter 13 Solutions
Biochemistry
Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...
Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Answers to all problems are at the end of this...Ch. 13 - Prob. 15PCh. 13 - Prob. 16PCh. 13 - Prob. 17PCh. 13 - Prob. 18PCh. 13 - Answers to all problems are at the end of this...
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