Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 13, Problem 11P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Quantitative Relationships Between Rate Constants to Calculate Km, Kinetic Efficiency (kcat/Km) and Vmax - III The citric acid cycle enzyme fumarase catalyzes the conversion of fumarate to form malate.

Fumarate+H 2 O malate The turnover number, kcat, for fumarase is 800/sec. The Km of fumarase for its substrate fumarate is 5 μ M .

a. In an experiment using 2 nanomole/mL of fumarase, what is Vmax?

b. The cellular concentration of fumarate is 47.5 μ M . What is v when [fumarate] = 47.5 μ M ?

c. What is the catalytic efficiency of fumarase?

d. Does fumarase approach "catalytic perfection"?

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